Journal article

Publication year: 1988

Pages: 5814-5821

Journal: Journal of Bacteriology

Volume number: 170

Issue number: 12

ISSN: 0021-9193

DOI Link: https://doi.org/10.1128/jb.170.12.5814-5821.1988

Publisher: American Society for Microbiology

Abstract: 

The polycistronic puf operon of Rhodobacter capsulatus encodes protein
components for the photosynthetic reaction center and one of the two
antenna complexes involved in the capture of light energy. We report
here that deletions within specific puf genes alter the synthesis and/or
assembly in the photosynthetic membranes of pigment-protein complexes
not affected genetically by the deletion. The pufX gene is required for
normal ratios of antenna complexes, and its deletion results in an
increase of membrane-bound light-harvesting I (LHI) complex-specific
proteins. Expression of pufQ in strains deleted for the genes encoding
the LHI and the photosynthetic reaction center (RC) yields a novel A868
peak that has not been associated with any of the pigment-protein
complexes described previously. While deletions in the RC-coding region
resulted in decreased LHI absorbance, no quantitative alteration in
membrane-bound LHI protein was observed, suggesting that an intact RC
complex is required for correct assembly of LHI in the membrane.

Harvard Citation style: Klug, G. and Cohen, S. (1988) Pleiotropic effects of localized Rhodobacter capsulatus puf operon deletions on production of light-absorbing pigment-protein complexes, Journal of Bacteriology, 170(12), pp. 5814-5821. https://doi.org/10.1128/jb.170.12.5814-5821.1988

APA Citation style: Klug, G., & Cohen, S. (1988). Pleiotropic effects of localized Rhodobacter capsulatus puf operon deletions on production of light-absorbing pigment-protein complexes. Journal of Bacteriology. 170(12), 5814-5821. https://doi.org/10.1128/jb.170.12.5814-5821.1988