Journalartikel

Jahr der Veröffentlichung: 2010

Seiten: 2791-2795

Zeitschrift: FEBS Letters

Bandnummer: 584

Heftnummer: 13

ISSN: 1873-3468

Open Access Status: Bronze

DOI Link: https://doi.org/10.1016/j.febslet.2010.05.013

Verlag: Wiley

Abstract: 
We studied the cellular localization of the archaeal exosome, an RNA-processing protein complex containing orthologs of the eukaryotic proteins Rrp41, Rrp42, Rrp4 and Csl4, and an archaea-specific subunit annotated as DnaG. Fractionation of cell-free extracts of Sulfolobus solfataricus in sucrose density gradients revealed that DnaG and the active-site comprising subunit Rrp41 are enriched together with surface layer proteins in a yellow colored ring, implicating that the exosome is membrane-bound. In accordance with this assumption, DnaG and Rrp41 were detected at the periphery of the cell by immunofluorescence microscopy. Our finding suggests that RNA processing in Archaea is spatially organized.

Harvard-Zitierstil: Roppelt, V., Hobel, C., Albers, S., Lassek, C., Schwarz, H., Klug, G., et al. (2010) The archaeal exosome localizes to the membrane, FEBS Letters, 584(13), pp. 2791-2795. https://doi.org/10.1016/j.febslet.2010.05.013

APA-Zitierstil: Roppelt, V., Hobel, C., Albers, S., Lassek, C., Schwarz, H., Klug, G., & Evguenieva-Hackenberg, E. (2010). The archaeal exosome localizes to the membrane. FEBS Letters. 584(13), 2791-2795. https://doi.org/10.1016/j.febslet.2010.05.013