Journal article

Publication year: 2010

Pages: 2931-2936

Journal: FEBS Letters

Volume number: 584

Issue number: 13

ISSN: 0014-5793

Open access status: Bronze

DOI Link: https://doi.org/10.1016/j.febslet.2010.05.014

Publisher: Wiley

Abstract: 
We studied the substrate specificity of the exosome of Sulfolobus solfataricus using the catalytically active Rrp41-Rrp42-hexamer and complexes containing the RNA-binding subunits Rrp4 or Csl4. The conservation of both Rrp4 and Csl4 in archaeal and eukaryotic exosomes suggests specific functions for each of them. We found that they confer different specificities to the exosome: RNA with an A-poor 3'-end is degraded with higher efficiency by the Csl4-exosome, while the Rrp4-exosome strongly prefers poly(A)-RNA. High C-content and polyuridylation negatively influence RNA processing by all complexes, and, in contrast to the hexamer, the Rrp4-exosome prefers longer substrates. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

Harvard Citation style: Roppelt, V., Klug, G. and Evguenieva-Hackenberg, E. (2010) The evolutionarily conserved subunits Rrp4 and Csl4 confer different substrate specificities to the archaeal exosome, FEBS Letters, 584(13), pp. 2931-2936. https://doi.org/10.1016/j.febslet.2010.05.014

APA Citation style: Roppelt, V., Klug, G., & Evguenieva-Hackenberg, E. (2010). The evolutionarily conserved subunits Rrp4 and Csl4 confer different substrate specificities to the archaeal exosome. FEBS Letters. 584(13), 2931-2936. https://doi.org/10.1016/j.febslet.2010.05.014