Journal article

ArcS, the Cognate Sensor Kinase in an Atypical Arc System of Shewanella oneidensis MR-1


Authors listLassak, J; Henche, AL; Binnenkade, L; Thormann, KM

Publication year2010

Pages3263-3274

JournalApplied and Environmental Microbiology

Volume number76

Issue number10

ISSN0099-2240

DOI Linkhttps://doi.org/10.1128/AEM.00512-10

PublisherAmerican Society for Microbiology


Abstract
The availability of oxygen is a major environmental factor for many microbes, in particular for bacteria such as Shewanella species, which thrive in redox-stratified environments. One of the best-studied systems involved in mediating the response to changes in environmental oxygen levels is the Arc two-component system of Escherichia coli, consisting of the sensor kinase ArcB and the cognate response regulator ArcA. An ArcA ortholog was previously identified in Shewanella, and as in Escherichia coli, Shewanella ArcA is involved in regulating the response to shifts in oxygen levels. Here, we identified the hybrid sensor kinase SO_0577, now designated ArcS, as the previously elusive cognate sensor kinase of the Arc system in Shewanella oneidensis MR-1. Phenotypic mutant characterization, transcriptomic analysis, protein-protein interaction, and phosphotransfer studies revealed that the Shewanella Arc system consists of the sensor kinase ArcS, the single phosphotransfer domain protein HptA, and the response regulator ArcA. Phylogenetic analyses suggest that HptA might be a relict of ArcB. Conversely, ArcS is substantially different with respect to overall sequence homologies and domain organizations. Thus, we speculate that ArcS might have adopted the role of ArcB after a loss of the original sensor kinase, perhaps as a consequence of regulatory adaptation to a redox-stratified environment.



Citation Styles

Harvard Citation styleLassak, J., Henche, A., Binnenkade, L. and Thormann, K. (2010) ArcS, the Cognate Sensor Kinase in an Atypical Arc System of Shewanella oneidensis MR-1, Applied and Environmental Microbiology, 76(10), pp. 3263-3274. https://doi.org/10.1128/AEM.00512-10

APA Citation styleLassak, J., Henche, A., Binnenkade, L., & Thormann, K. (2010). ArcS, the Cognate Sensor Kinase in an Atypical Arc System of Shewanella oneidensis MR-1. Applied and Environmental Microbiology. 76(10), 3263-3274. https://doi.org/10.1128/AEM.00512-10


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