Journal article
Authors list: Jasiak, AJ; Hartmann, H; Karakasili, E; Kalocsay, M; Flatley, A; Kremmer, E; Sträßer, K; Martin, DE; Söding, J; Cramer, P
Publication year: 2008
Pages: 26423-26427
Journal: Journal of Biological Chemistry
Volume number: 283
Issue number: 39
ISSN: 0021-9258
DOI Link: https://doi.org/10.1074/jbc.M803237200
Publisher: Elsevier
Abstract:
Yeast RNA polymerase (Pol) II consists of a 10-subunit core enzyme and the Rpb4/7 subcomplex, which is dispensable for catalytic activity and dissociates in vitro. To investigate whether Rpb4/7 is an integral part of DNA-associated Pol II in vivo, we used chromatin immunoprecipitation coupled to high resolution tiling microarray analysis. We show that the genome-wide occupancy profiles for Rpb7 and the core subunit Rpb3 are essentially identical. Thus, the complete Pol II associates with DNA in vivo, consistent with functional roles of Rpb4/7 throughout the transcription cycle.
Citation Styles
Harvard Citation style: Jasiak, A., Hartmann, H., Karakasili, E., Kalocsay, M., Flatley, A., Kremmer, E., et al. (2008) Genome-associated RNA polymerase II includes the dissociable Rpb4/7 subcomplex, Journal of Biological Chemistry, 283(39), pp. 26423-26427. https://doi.org/10.1074/jbc.M803237200
APA Citation style: Jasiak, A., Hartmann, H., Karakasili, E., Kalocsay, M., Flatley, A., Kremmer, E., Sträßer, K., Martin, D., Söding, J., & Cramer, P. (2008). Genome-associated RNA polymerase II includes the dissociable Rpb4/7 subcomplex. Journal of Biological Chemistry. 283(39), 26423-26427. https://doi.org/10.1074/jbc.M803237200
