Journal article

Publication year: 2008

Pages: 872-877

Journal: Genes & Development

Volume number: 22

Issue number: 7

ISSN: 0890-9369

DOI Link: https://doi.org/10.1101/gad.465108

Publisher: Cold Spring Harbor Laboratory Press

Abstract: 
A combination of crystallography, biochemistry, and gene expression analysis identifies the coactivator subcomplex Med8C/18/20 as a functionally distinct submodule of the Mediator head module. Med8C forms a conserved alpha-helix that tethers Med18/20 to the Mediator. Deletion of Med8C in vivo results in dissociation of Med18/20 from Mediator and in loss of transcription activity of extracts. Deletion of med8C, med18, or med20 causes similar changes in the yeast transcriptome, establishing Med8C/18/20 as a predominantly positive, gene-specific submodule required for low transcription levels of nonactivated genes, including conjugation genes. The presented structure-based system perturbation is superior to gene deletion analysis of gene regulation.

Harvard Citation style: Larivière, L., Seizl, M., van Wageningen, S., Röther, S., van de Pasch, L., Feldmann, H., et al. (2008) Structure-system correlation identifies a gene regulatory Mediator submodule, Genes & Development, 22(7), pp. 872-877. https://doi.org/10.1101/gad.465108

APA Citation style: Larivière, L., Seizl, M., van Wageningen, S., Röther, S., van de Pasch, L., Feldmann, H., Sträßer, K., Hahn, S., Holstege, F., & Cramer, P. (2008). Structure-system correlation identifies a gene regulatory Mediator submodule. Genes & Development. 22(7), 872-877. https://doi.org/10.1101/gad.465108