Journal article

Publication year: 2015

Pages: 30590-30597

Journal: Physical Chemistry Chemical Physics

Volume number: 17

Issue number: 45

ISSN: 1463-9076

Open access status: Hybrid

DOI Link: https://doi.org/10.1039/c5cp05454k

Publisher: Royal Society of Chemistry

Abstract: 
Although seminaphtorhodafluor (SNARF) dyes are already widely used to measure pH in cells and at biofilms, their synthesis has low yield and results in an unspecific position of a carboxy-group. The separation of 5'-and 6'-carboxy-SNARF reveals a pK(a) difference of 0.15, calling into question pH measurements with the (commercially available) mixture. Here we replace the bulky external dicarboxyphenyl ring with a propionate group and evaluate the spectral properties of the new derivative. Proceeding to the ethyl-iodoacetamide, covalent linkage to cysteine protein sites is achieved efficiently as shown with a cyanobacterial phytochrome, extending the scarce application of SNARF in bio-labelling in the current literature. Application in fluorescence lifetime imaging is demonstrated both with the lifetime-based and ratiometric-yield method.

Harvard Citation style: Richter, C., Schneider, C., Quick, M., Volz, P., Mahrwald, R., Hughes, J., et al. (2015) Dual-fluorescence pH probe for bio-labelling, Physical Chemistry Chemical Physics, 17(45), pp. 30590-30597. https://doi.org/10.1039/c5cp05454k

APA Citation style: Richter, C., Schneider, C., Quick, M., Volz, P., Mahrwald, R., Hughes, J., Dick, B., Alexiev, U., & Ernsting, N. (2015). Dual-fluorescence pH probe for bio-labelling. Physical Chemistry Chemical Physics. 17(45), 30590-30597. https://doi.org/10.1039/c5cp05454k