Journal article

Publication year: 2010

Pages: 161-170

Journal: The Journal of Steroid Biochemistry and Molecular Biology

Volume number: 119

Issue number: 3-5

ISSN: 0960-0760

DOI Link: https://doi.org/10.1016/j.jsbmb.2010.02.020

Publisher: Elsevier

Abstract: 
Although beta-boldenone (bBol) used to be a marker of illegal steroid administration in calves, its endogenous formation has recently been demonstrated in these vertebrates However, research on the pathway leading to bBol remains scarce This study shows the usefulness of in vivo invertebrate models as alternatives to vertebrate animal experiments, using Neomysis integer and Lucilia sericata. In accordance with vertebrates, androstenedione (AED) was the main metabolite of beta-testosterone (bT) produced by these invertebrates, and bBol was also frequently detected Moreover. in vitro experiments using feed-borne fungi and microsomes were useful to perform the pathway from bT to bBol Even the conversion of phytosterols into steroids was shown in vitro Both in vivo and in vitro. the conversion of bT into bBol could be demonstrated in this study. Metabolism of phytosterols by feed-borne fungi may be of particular importance to explain the endogenous bBol-formation by cattle To the best of our knowledge, it is the first time the latter pathway is described in literature.

Harvard Citation style: Verheyden, K., Noppe, H., Zorn, H., Van Immerseel, F., Bussche, J., Wille, K., et al. (2010) Endogenous boldenone-formation in cattle: Alternative invertebrate organisms to elucidate the enzymatic pathway and the potential role of edible fungi on cattle's feed, The Journal of Steroid Biochemistry and Molecular Biology, 119(3-5), pp. 161-170. https://doi.org/10.1016/j.jsbmb.2010.02.020

APA Citation style: Verheyden, K., Noppe, H., Zorn, H., Van Immerseel, F., Bussche, J., Wille, K., Bekaert, K., Janssen, C., De Brabander, H., & Vanhaecke, L. (2010). Endogenous boldenone-formation in cattle: Alternative invertebrate organisms to elucidate the enzymatic pathway and the potential role of edible fungi on cattle's feed. The Journal of Steroid Biochemistry and Molecular Biology. 119(3-5), 161-170. https://doi.org/10.1016/j.jsbmb.2010.02.020