Phytochrome as Molecular Machine: Revealing Chromophore Action during the Pfr -> Pr Photoconversion by Magic-Angle Spinning NMR Spectroscopy - Research portal of Justus Liebig University Giessen:

Journal article

Phytochrome as Molecular Machine: Revealing Chromophore Action during the Pfr -> Pr Photoconversion by Magic-Angle Spinning NMR Spectroscopy

Authors list: Rohmer, T; Lang, C; Bongards, C; Gupta, KBSS; Neugebauer, J; Hughes, J; Gartner, W; Matysik, J

Publication year: 2010

Pages: 4431-4437

Journal: Journal of the American Chemical Society

Volume number: 132

Issue number: 12

ISSN: 0002-7863

DOI Link: https://doi.org/10.1021/ja9108616

Publisher: American Chemical Society

Abstract:
The cyanobacterial phytochrome Cph1 can be photoconverted between two thermally stable states, Pr and Pfr. The photochemically induced Pfr -> Pr back-reaction has been followed at low temperature by magic-angle spinning (MAS) NMR spectroscopy, allowing two intermediates, Lumi-F and Meta-F, to be trapped. Employing uniformly C-13- and N-15-labeled open-chain tetrapyrrole chromophores, all four states Pfr, Lumi-F, Meta-F, and Pr-have been structurally characterized. In the first step, the double bond. photoisomerization forming Lumi-F occurs. The second step, the transformation to Meta-F, is driven by the release of the mechanical tension. This process leads to the break of the hydrogen bond of the ring D nitrogen to Asp-207 and triggers signaling. The third step is protonically driven allowing the hydrogen-bonding interaction of the ring D nitrogen to be restored. Compared to the forward reaction, the order of events is changed, probably caused by the different properties of the hydrogen bonding partners of N24, leading to the directionality of the photocycle.

Citation Styles

Harvard Citation style: Rohmer, T., Lang, C., Bongards, C., Gupta, K., Neugebauer, J., Hughes, J., et al. (2010) Phytochrome as Molecular Machine: Revealing Chromophore Action during the Pfr -> Pr Photoconversion by Magic-Angle Spinning NMR Spectroscopy, Journal of the American Chemical Society, 132(12), pp. 4431-4437. https://doi.org/10.1021/ja9108616

APA Citation style: Rohmer, T., Lang, C., Bongards, C., Gupta, K., Neugebauer, J., Hughes, J., Gartner, W., & Matysik, J. (2010). Phytochrome as Molecular Machine: Revealing Chromophore Action during the Pfr -> Pr Photoconversion by Magic-Angle Spinning NMR Spectroscopy. Journal of the American Chemical Society. 132(12), 4431-4437. https://doi.org/10.1021/ja9108616

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