Journal article

Publication year: 2007

Pages: 474-481

Journal: Proteomics: Proteomics and Systems Biology

Volume number: 7

Issue number: 3

ISSN: 1615-9853

Open access status: Green

DOI Link: https://doi.org/10.1002/pmic.200600719

Publisher: Wiley

Abstract: 
Leptomeningeal metastasis (LM) is a devastating complication occurring in 5% of breast cancer patients. However, the current 'gold standard of diagnosis, namely microscopic examination of the cerebrospinal fluid (CSF), is false-negative in 25% of patients at the first lumbar puncture. In a previous study, we analyzed a set of 151 CSF samples (tryptic digests) by MALDI-TOFand detected peptide masses that were differentially expressed in breast cancer patients with LM. In the present study, we obtain for a limited number of samples exact masses for these peptides by MALDI-FTICR MS measurements. Identification of these peptides was performed by electrospray FTICR NIS after separation by nano-scale LC. The database results were confirmed by targeted high mass accuracy measurements of the fragment ions in the FTICR cell. The combination of automated high-throughput MALDI-TOF measurements and analysis by FTICR NIS leads to the identification of 17 peptides corresponding to 9 proteins. These include proteins that are operative in host-disease interaction, inflammation and immune defense (serotransferrin, alpha 1-antichymotrypsin, hemopexin, haptoglobin and transthyretin). Several of these proteins have been mentioned in the literature in relation to cancer. The identified proteins alpha 1-antichymotrypsin and apolipoprotein E have been described in relation to Alzheimer's disease and brain cancer.

Harvard Citation style: Römpp, A., Dekker, L., Taban, I., Jenster, G., Boogerd, W., Bonfrer, H., et al. (2007) Identification of leptomeningeal metastasis-related proteins in cerebrospinal fluid of patients with breast cancer by a combination of MALDI-TOF, MALDI-FTICR and nanoLC-FTICR MS, Proteomics: Proteomics and Systems Biology, 7(3), pp. 474-481. https://doi.org/10.1002/pmic.200600719

APA Citation style: Römpp, A., Dekker, L., Taban, I., Jenster, G., Boogerd, W., Bonfrer, H., Spengler, B., Heeren, R., Smitt, P., & Luider, T. (2007). Identification of leptomeningeal metastasis-related proteins in cerebrospinal fluid of patients with breast cancer by a combination of MALDI-TOF, MALDI-FTICR and nanoLC-FTICR MS. Proteomics: Proteomics and Systems Biology. 7(3), 474-481. https://doi.org/10.1002/pmic.200600719