Journalartikel

Jahr der Veröffentlichung: 2008

Seiten: 43-48

Zeitschrift: Journal of Chromatography A

Bandnummer: 1185

Heftnummer: 1

ISSN: 0021-9673

DOI Link: https://doi.org/10.1016/j.chroma.2007.12.045

Verlag: Elsevier

Abstract: 
A high-performance liquid chromatographic (HPLC) method was developed for the determination of N-nitrosodiethanolamine (NDELA), which is a non-volatile N-nitrosoamine. In this method, sodium 1-octanesulfonate has been used as an ion complexation agent for NDELA. The resulting complex was analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC) using a Eurospher-100C18 column (250 x 4.6 mm, 5 mu m), water/acetonitrile (95/5, v/v) and UV detection at 234 nm. The detector response was linear in the range 0.03-10.00 mu g mL(-1) and the limit of detection was 0.01 mu g mL(-1). The complexation of the NDELA and sodium 1-octanesulfonate was confirmed by negative ion electrospray ionization mass spectrometry at m/z 327 [M-Na](-) and also MS/MS measurements of the resulting fragments. For real sample analyses, NDELA was measured in cosmetic products after clean up by solid-phase extraction (SPE) with C-18 cartridge for water-soluble samples and liquid-liquid extraction (LLE) by methylene chloride for less water-soluble samples. The average values for NDELA recovery by SPE and LLE were 86.9 and 51.8% and relative standard deviations (RSDs%) were 0.9 and 5.6%, respectively. The presented method is easy to use, robust and can be implemented on any reversed-phase HPLC system with UV detection.

Harvard-Zitierstil: Ghassemnpour, A., Abbaci, M., Talebpour, Z., Spengler, B. and Römpp, A. (2008) Monitoring of N-nitrosodiethanolamine in cosmetic products by ion-pair complex liquid chromatography and identification with negative ion electrospray ionization mass spectrometry, Journal of Chromatography A, 1185(1), pp. 43-48. https://doi.org/10.1016/j.chroma.2007.12.045

APA-Zitierstil: Ghassemnpour, A., Abbaci, M., Talebpour, Z., Spengler, B., & Römpp, A. (2008). Monitoring of N-nitrosodiethanolamine in cosmetic products by ion-pair complex liquid chromatography and identification with negative ion electrospray ionization mass spectrometry. Journal of Chromatography A. 1185(1), 43-48. https://doi.org/10.1016/j.chroma.2007.12.045