Journal article

An aryl-alcohol oxidase of Pleurotus sapidus: heterologous expression, characterization, and application in a 2-enzyme system


Authors listGalperin, I; Javeed, A; Luig, H; Lochnit, G; Rühl, M

Publication year2016

Pages8021-8030

JournalApplied Microbiology and Biotechnology

Volume number100

Issue number18

ISSN0175-7598

DOI Linkhttps://doi.org/10.1007/s00253-016-7567-8

PublisherSpringer


Abstract
Aryl-alcohol oxidases (AAOs) are enzymes supporting the degradation of lignin by fungal derived class II peroxidases produced by white-rot fungi. AAOs are able to generate H2O2 as a by-product via oxidation of an aryl-alcohol into its correspondent aldehyde. In this study, an AAO was heterologously expressed in a basidiomycete host for the first time. The gene for an AAO of the white-rot fungus Pleurotus sapidus, a close relative to the oyster mushroom Pleurotus ostreatus, was cloned into an expression vector and put under control of the promotor of the glyceraldehyde-3-phosphate dehydrogenase gene 2 (gpdII) of the button mushroom Agaricus bisporus. The expression vector was transformed into the model basidiomycete Coprinopsis cinerea, and several positive transformants were obtained. The best producing transformants were grown in shake-flasks and in a stirred tank reactor reaching enzymatic activities of up to 125 U L-1 using veratryl alcohol as a substrate. The purified AAO was biochemically characterized and compared to the previously described native and recombinant AAOs from other Pleurotus species. In addition, a two-enzyme system comprising a dye-decolorizing peroxidase (DyP) from Mycetinis scorodonius and the P. sapidus AAO was successfully employed to bleach the anthraquinone dye Reactive Blue 5.



Citation Styles

Harvard Citation styleGalperin, I., Javeed, A., Luig, H., Lochnit, G. and Rühl, M. (2016) An aryl-alcohol oxidase of Pleurotus sapidus: heterologous expression, characterization, and application in a 2-enzyme system, Applied Microbiology and Biotechnology, 100(18), pp. 8021-8030. https://doi.org/10.1007/s00253-016-7567-8

APA Citation styleGalperin, I., Javeed, A., Luig, H., Lochnit, G., & Rühl, M. (2016). An aryl-alcohol oxidase of Pleurotus sapidus: heterologous expression, characterization, and application in a 2-enzyme system. Applied Microbiology and Biotechnology. 100(18), 8021-8030. https://doi.org/10.1007/s00253-016-7567-8


Last updated on 2025-21-05 at 15:03