E-paper

Publication year: 2017

Journal: Protocol Exchange

DOI Link: https://doi.org/10.1038/protex.2017.103

Publisher: Research Square

Abstract: 

Mass spectrometry imaging (MSI) is a profiling technique that allows to visualize molecular information from various sample surfaces. In this protocol, we provide the experimental details for surface profiling of three-dimensional (3D) objects, i.e. rough surfaces using LDI or MALDI MSI at ambient pressure. This procedure is applicable to various objects, such as inorganic surfaces, intact plants, insects and imperfect tissue sections. We provide a protocol for matrix application, data analysis and data visualization for the use with a high-resolution atmospheric pressure MALDI MSI source. This protocol is intended for 3D sample imaging with down to 10 µm lateral resolution and about 1.5 µm height resolution. As an example, various substance classes on plant, insect and coin surfaces were imaged. The data analysis, including MS image generation, generation of topographic images and the combination to a multi-dimensional data visualization are described and discussed in this protocol. Compound assignment is based on accurate mass measurements (mass error less than 2 ppm) using a high mass resolution (R=140.000 at m/z=200) orbital trapping mass spectrometer.

Harvard Citation style: Spengler, B., Kompauer, M. and Heiles, S. (2017) Chemical and topographical 3D surface profiling using atmospheric pressure LDI and MALDI MS imaging [Preprint]. Protocol Exchange. https://doi.org/10.1038/protex.2017.103

APA Citation style: Spengler, B., Kompauer, M., & Heiles, S. (2017). Chemical and topographical 3D surface profiling using atmospheric pressure LDI and MALDI MS imaging. Protocol Exchange. https://doi.org/10.1038/protex.2017.103