Contribution in an anthology

Appeared in: Light sensing in plants

Editor list: Masamitsu, W; Shimazaki, K; Iino, M

Publication year: 2005

Pages: 103-110

ISBN: 978-4-431-24002-0

eISBN: 978-4-431-27092-8

DOI Link: https://doi.org/10.1007/4-431-27092-2_11

Abstract: 

The lowly moss Physcomitrella patens
is an excellent model organism for modern molecular physiology. The
protonemal filaments are haploid, displaying the phenotype of a genetic
lesion immediately after mutagenesis (selfing is possible but
unnecessary). Filament cells are amenable to a wide range of cell
biological methods including microinjection and, uniquely amongst
plants, gene targeting via homologous recombination. Thus a Physcomitrella
gene can be cloned, disrupted in situ and the mutant filament-together
with its phenotype—regenerated within a couple of weeks. Protonemata are
especially interesting photobiologically as they use phytochrome to
steer their direction of growth in relation to light (phototropism). As
we shall see, according to the fashionable view of phytochrome molecular
action, this is simply not possible. We hope to resolve this
self-contradictory situation using the power of the Physcomitrella system.

Harvard Citation style: Hughes, J., Brücker, G., Repp, A., Zeidler, M. and Mittmann, F. (2005) Phytochromes and Functions: Studies Using Gene Targeting in Physcomitrella, in Masamitsu, W., Shimazaki, K. and Iino, M. (eds.) Light sensing in plants. Tokyo: Springer, pp. 103-110. https://doi.org/10.1007/4-431-27092-2_11

APA Citation style: Hughes, J., Brücker, G., Repp, A., Zeidler, M., & Mittmann, F. (2005). Phytochromes and Functions: Studies Using Gene Targeting in Physcomitrella. In Masamitsu, W., Shimazaki, K., & Iino, M. (Eds.), Light sensing in plants (pp. 103-110). Springer. https://doi.org/10.1007/4-431-27092-2_11