Journalartikel

Jahr der Veröffentlichung: 2007

Seiten: 18660-18665

Zeitschrift: Proceedings of the National Academy of Sciences

Bandnummer: 104

Heftnummer: 47

ISSN: 0027-8424

Open Access Status: Green

DOI Link: https://doi.org/10.1073/pnas.0705939104

Verlag: National Academy of Sciences

Abstract: 
Lack of the IL-1 receptor accessory protein (IL-1RAcP) abrogates responses to IL-33 and IL-1 in the mouse thymoma clone EL-4 D6/76 cells. Reconstitution with full-length lL-1RAcP is sufficient to restore responsiveness to IL-33 and IL-1. IL-33 activates IL-1 receptor-associated kinase-1, cJun-N-terminal kinase, and the NF-kappa B pathway in an IL-1RAcP-dependent manner and results in IL-2 release. IL-33 is able to induce the release of proinflammatory cytokines in bone marrow-derived (BMD) mast cells, indicating that IL-33 may have a proinflammatory potential like its relatives IL-1 and IL-18, in addition to its Th2-skewing properties in the adaptive response described previously. Blocking of murine IL-1RAcP with the neutralizing antibody 4C5 inhibits response of mouse thymoma cells and BMD mast cells to IL-33. The interaction of either membrane-bound or soluble forms of lL-1RAcP and IL-33R alpha-chain depends on the presence of IL-33, as demonstrated by coimmuno-precipitation assays. These data demonstrate that IL-1RAcP is indispensable for IL-33 signaling. Furthermore, they suggest that IL-1RAcP is used by more than one alpha-chain of the IL-1 receptor family and thus may resemble a common beta-chain of that family.

Harvard-Zitierstil: Ali, S., Hubert, M., Kollewe, C., Bischoff, S., Falk, W. and Martin, M. (2007) IL-1 receptor accessory protein is essential for IL-33-induced activation of T lymphocytes and mast cells, Proceedings of the National Academy of Sciences, 104(47), pp. 18660-18665. https://doi.org/10.1073/pnas.0705939104

APA-Zitierstil: Ali, S., Hubert, M., Kollewe, C., Bischoff, S., Falk, W., & Martin, M. (2007). IL-1 receptor accessory protein is essential for IL-33-induced activation of T lymphocytes and mast cells. Proceedings of the National Academy of Sciences. 104(47), 18660-18665. https://doi.org/10.1073/pnas.0705939104