Journal article

Publication year: 1996

Pages: 652-663

Journal: RNA

Volume number: 2

Issue number: 7

ISSN: 1355-8382

URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1369404/

Publisher: Cold Spring Harbor Laboratory Press

Abstract: 
Chlamydomonas reinhardtii strains harboring deletions of the chloroplast atpB 3' inverted repeat (IR) are weakly phototrophic due to reduced accumulation of discrete atpB transcripts and the chloroplast ATPase beta-subunit protein. A sequence of 18 guanosine residues, which can impede a 3' --> 5' exoribonuclease in vitro, is able to substitute for the atpB IR in vivo. Strains containing the poly-guanosine tract in place of the atpB 3' IR are phototrophic and accumulate near wild-type levels of discrete atpB transcripts and the ATPase beta-subunit protein. Because these atpS transcripts contain the 18 guanosine residues, and the poly-guanosine tract is not a terminator of transcription, the accumulation of discrete atpB transcripts is likely the result of impediment of 3' --> 5' exoribonuclease activity. These findings support a model in which atpB transcripts lacking the 3' IR are degraded by 3' --> 5' exoribonuclease activity, and demonstrate that the poly-guanosine tract can be used to study chloroplast RNA metabolism in vivo.

Harvard Citation style: Drager, R., Zeidler, M., Simpson, G. and Stern, D. (1996) A chloroplast transcript lacking the 3' inverted repeat is degraded by 3'->5' exoribonuclease activity, RNA, 2(7), pp. 652-663. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1369404/

APA Citation style: Drager, R., Zeidler, M., Simpson, G., & Stern, D. (1996). A chloroplast transcript lacking the 3' inverted repeat is degraded by 3'->5' exoribonuclease activity. RNA. 2(7), 652-663. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1369404/