Journalartikel

Jahr der Veröffentlichung: 2009

Seiten: 38-49

Zeitschrift: Journal of Biotechnology

Bandnummer: 142

Heftnummer: 1

ISSN: 0168-1656

eISSN: 1873-4863

DOI Link: https://doi.org/10.1016/j.jbiotec.2009.02.010

Verlag: Elsevier

Abstract: 
The phylogenetic structure of the microbial community residing in a fermentation sample from a production-scale biogas plant fed with maize silage. green rye and liquid manure was analysed by an integrated approach using clone library sequences and metagenome sequence data obtained by 454-pyrosequencing. Sequencing of 109 clones from a bacterial and an archaeal 165-rDNA amplicon library revealed that the obtained nucleotide sequences are similar but not identical to 165-rDNA database sequences derived from different anaerobic environments including digestors and bioreactors. Most of the bacterial 165-rDNA sequences could be assigned to the phylum Firmicutes with the most abundant class Clostridia and to the class Bacteroidetes, whereas most archaeal 165-rDNA sequences cluster close to the methanogen Methanoculleus bourgensis. Further sequences of the archaeal library most probably represent so far non-characterised species within the genus Methanoculleus. A similar result derived from phylogenetic analysis of mcrA clone sequences. The mcrA gene product encodes the a-subunit of methyl-coenzyme-M reductase involved in the final step of methanogenesis. BLASTn analysis applying stringent settings resulted in assignment of 165-rDNA metagenome sequence reads to 62 165-rDNA amplicon sequences thus enabling frequency of abundance estimations for 165-rDNA clone library sequences. Ribosomal Database Project (RDP) Classifier processing of metagenome 165-rDNA reads revealed abundance of the phyla Firmicutes, Bacteroidetes and Euryarchaeota and the orders Clostridiales, Bacteroidales and Methanomicrobiales. Moreover, a large fraction of 165-rDNA metagenome reads could not be assigned to lower taxonomic ranks, demonstrating that numerous microorganisms in the analysed fermentation sample of the biogas plant are still unclassified or unknown.

Harvard-Zitierstil: Kröber, M., Bekel, T., Diaz, N., Goesmann, A., Jaenicke, S., Krause, L., et al. (2009) Phylogenetic characterization of a biogas plant microbial community integrating clone library 16S-rDNA sequences and metagenome sequence data obtained by 454-pyrosequencing, Journal of Biotechnology, 142(1), pp. 38-49. https://doi.org/10.1016/j.jbiotec.2009.02.010

APA-Zitierstil: Kröber, M., Bekel, T., Diaz, N., Goesmann, A., Jaenicke, S., Krause, L., Miller, D., Runte, K., Viehöver, P., Pühler, A., & Schlüter, A. (2009). Phylogenetic characterization of a biogas plant microbial community integrating clone library 16S-rDNA sequences and metagenome sequence data obtained by 454-pyrosequencing. Journal of Biotechnology. 142(1), 38-49. https://doi.org/10.1016/j.jbiotec.2009.02.010