Journal article

Publication year: 2013

Pages: 2101-2114

Journal: Bioanalysis

Volume number: 5

Issue number: 17

ISSN: 1757-6180

DOI Link: https://doi.org/10.4155/bio.13.187

Publisher: Taylor and Francis Group

Abstract: 
The influence of matrix effects in LC-MS/MS analysis of biological samples can be enormous and has to be evaluated during method development. Phospholipids, which are present in considerable quantities in biological fluids, are supposed to cause matrix effects when co-eluting with analytes. Therefore, the reduction of phospholipids should lead to the minimization of matrix effects. METHODOLOGY & RESULTS: Here, a polymeric reversed-phase (PRP) SPE cartridge was compared with a combination of mixed-mode-anion-exchange (MAX) and mixed-mode-cation-exchange (MCX) SPE cartridges regarding elimination of matrix effects during sample clean-up. For evaluation of matrix effects post-column infusion experiments were performed. Phospholipid amount in the sample extract and matrix effects are enhanced using PRP in contrast to the combination of MAX/MCX.
For an efficient elimination of phospholipids during sample preparation and to improve method accuracy and precision it is advisable to use a combination of MAX/MCX SPE cartridges.

Harvard Citation style: Janusch, F., Kalthoff, L., Hamscher, G. and Mohring, S. (2013) Evaluation and subsequent minimization of matrix effects caused by phospholipids in LC-MS analysis of biological samples, Bioanalysis, 5(17), pp. 2101-2114. https://doi.org/10.4155/bio.13.187

APA Citation style: Janusch, F., Kalthoff, L., Hamscher, G., & Mohring, S. (2013). Evaluation and subsequent minimization of matrix effects caused by phospholipids in LC-MS analysis of biological samples. Bioanalysis. 5(17), 2101-2114. https://doi.org/10.4155/bio.13.187