Journalartikel

Jahr der Veröffentlichung: 1998

Seiten: 188-193

Zeitschrift: Journal of Peptide Research

Bandnummer: 51

Heftnummer: 3

ISSN: 1397-002X

Verlag: Blackwell Publishing Ltd

Abstract: 
Ln this paper, we report on the identification of an unexpected acylation that occurred on the solid phase when a peptide containing an unprotected lysyl and a tosyl-protected arginyl residue was treated with a large excess of isonicotinyl p-nitrophenylcarbonate. NMR and matrix-assisted laser desorption/ionisation -post-source decay analysis of the purified peptide demonstrated the presence of one extra isonicotinyloxycarbonyl (iNoc) group located on the omega nitrogen atom of the arginine which was adjacent to the Lys(iNoc). The desired peptide was obtained by quantitative removal of the unwanted iNoc group during a brief treatment with diluted aqueous hydrazine.

Harvard-Zitierstil: Melnyk, O., Chaurand, P., Rommens, C., Drobecq, H., Wieruszeski, J., Spengler, B., et al. (1998) Identification of a sequence-dependent reversible acylation of tosylarginine in a peptidyl-resin reacted with isonicotinyl p-nitrophenylcarbonate, Journal of Peptide Research, 51(3), pp. 188-193

APA-Zitierstil: Melnyk, O., Chaurand, P., Rommens, C., Drobecq, H., Wieruszeski, J., Spengler, B., & Gras-Masse, H. (1998). Identification of a sequence-dependent reversible acylation of tosylarginine in a peptidyl-resin reacted with isonicotinyl p-nitrophenylcarbonate. Journal of Peptide Research. 51(3), 188-193.