Deep sequencing of naupliar-, cyprid- and adult-specific normalised Expressed Sequence Tag (EST) libraries of the acorn barnacle Balanus amphitrite - Research portal of Justus Liebig University Giessen:

Journal article

Deep sequencing of naupliar-, cyprid- and adult-specific normalised Expressed Sequence Tag (EST) libraries of the acorn barnacle Balanus amphitrite

Authors list: De Gregoris, TB; Rupp, O; Klages, S; Knaust, F; Bekel, T; Kube, M; Burgess, JG; Arnone, MI; Goesmann, A; Reinhardt, R; Clare, AS

Publication year: 2011

Pages: 367-374

Journal: Biofouling: The Journal of Bioadhesion and Biofilm Research

Volume number: 27

Issue number: 4

ISSN: 0892-7014

eISSN: 1029-2454

DOI Link: https://doi.org/10.1080/08927014.2011.577211

Publisher: Taylor and Francis Group

Abstract:
In order to improve the genetic characterisation of the barnacle Balanus amphitrite, normalised EST libraries for the developmental stages, viz. nauplius (a mix of instars I and II), cyprid and adult, were generated. The libraries were sequenced independently using 454 technologies and 575,666 reads were generated. For adults, 4843 unique isotigs were estimated and 6754 and 7506 in the cyprid and naupliar stage, respectively. It was found that some of the previously proposed cyprid-specific bcs genes were also expressed during the naupliar and adult stage. Furthermore, as lectins have been hypothesised to influence settlement cue recognition in barnacles, the database was searched for lectin-like isotigs. Two proteins, uniquely expressed in either the cyprid or the adult stage, matched a mannose receptor, and their nucleotide sequences were 33% and 31% identical to a lectin (BRA-3) isolated from Megabalanus rosa. Further characterisation of these genes may suggest their involvement in settlement.

Authors/Editors

- Uni.-Prof. Dr. Alexander Goesmann

Citation Styles

Harvard Citation style: De Gregoris, T., Rupp, O., Klages, S., Knaust, F., Bekel, T., Kube, M., et al. (2011) Deep sequencing of naupliar-, cyprid- and adult-specific normalised Expressed Sequence Tag (EST) libraries of the acorn barnacle Balanus amphitrite, Biofouling: The Journal of Bioadhesion and Biofilm Research, 27(4), pp. 367-374. https://doi.org/10.1080/08927014.2011.577211

APA Citation style: De Gregoris, T., Rupp, O., Klages, S., Knaust, F., Bekel, T., Kube, M., Burgess, J., Arnone, M., Goesmann, A., Reinhardt, R., & Clare, A. (2011). Deep sequencing of naupliar-, cyprid- and adult-specific normalised Expressed Sequence Tag (EST) libraries of the acorn barnacle Balanus amphitrite. Biofouling: The Journal of Bioadhesion and Biofilm Research. 27(4), 367-374. https://doi.org/10.1080/08927014.2011.577211