Journal article

Trans-spliceosomal U6 RNAs of Crithidia fasciculata and Leptomonas seymouri: deviation from the conserved ACAGAG sequence and potential base pairing with spliced leader RNA.


Authors listXu, GL; Wieland, B; Bindereif, A

Publication year1994

Pages4565-4570

JournalMolecular and Cellular Biology

Volume number14

Issue number7

ISSN0270-7306

eISSN1098-5549

DOI Linkhttps://doi.org/10.1128/MCB.14.7.4565

PublisherTaylor and Francis Group


Abstract
U6 RNA genes from the trypanosomatids Crithidia fasciculata and Leptomonas seymouri have been isolated and sequenced. As in Trypanosoma brucei, the U6 RNA genes in both C. fasciculata and L. seymouri are arranged in close linkage with upstream tRNA genes. The U6 RNA sequences from C. fasciculata and L. seymouri deviate in five and three positions, respectively, from the published T. brucei sequence. Interestingly, both C. fasciculata U6 RNA genes carry a C-->T change at the second position of the ACAGAG hexanucleotide sequence, which is important for splicing function and has been considered phylogenetically invariable. A compensatory base change of the C. fasciculata spliced leader RNA at the highly conserved 5' splice site position +5, G-->A, suggests that an interaction between the 5' splice site region and U6 RNA recently proposed for the yeast cis-splicing system may also occur in trans splicing.



Citation Styles

Harvard Citation styleXu, G., Wieland, B. and Bindereif, A. (1994) Trans-spliceosomal U6 RNAs of Crithidia fasciculata and Leptomonas seymouri: deviation from the conserved ACAGAG sequence and potential base pairing with spliced leader RNA., Molecular and Cellular Biology, 14(7), pp. 4565-4570. https://doi.org/10.1128/MCB.14.7.4565

APA Citation styleXu, G., Wieland, B., & Bindereif, A. (1994). Trans-spliceosomal U6 RNAs of Crithidia fasciculata and Leptomonas seymouri: deviation from the conserved ACAGAG sequence and potential base pairing with spliced leader RNA.. Molecular and Cellular Biology. 14(7), 4565-4570. https://doi.org/10.1128/MCB.14.7.4565


Last updated on 2025-21-05 at 15:31