Journal article
Authors list: Xu, GL; Wieland, B; Bindereif, A
Publication year: 1994
Pages: 4565-4570
Journal: Molecular and Cellular Biology
Volume number: 14
Issue number: 7
ISSN: 0270-7306
eISSN: 1098-5549
DOI Link: https://doi.org/10.1128/MCB.14.7.4565
Publisher: Taylor and Francis Group
Abstract:
U6 RNA genes from the trypanosomatids Crithidia fasciculata and Leptomonas seymouri have been isolated and sequenced. As in Trypanosoma brucei, the U6 RNA genes in both C. fasciculata and L. seymouri are arranged in close linkage with upstream tRNA genes. The U6 RNA sequences from C. fasciculata and L. seymouri deviate in five and three positions, respectively, from the published T. brucei sequence. Interestingly, both C. fasciculata U6 RNA genes carry a C-->T change at the second position of the ACAGAG hexanucleotide sequence, which is important for splicing function and has been considered phylogenetically invariable. A compensatory base change of the C. fasciculata spliced leader RNA at the highly conserved 5' splice site position +5, G-->A, suggests that an interaction between the 5' splice site region and U6 RNA recently proposed for the yeast cis-splicing system may also occur in trans splicing.
Citation Styles
Harvard Citation style: Xu, G., Wieland, B. and Bindereif, A. (1994) Trans-spliceosomal U6 RNAs of Crithidia fasciculata and Leptomonas seymouri: deviation from the conserved ACAGAG sequence and potential base pairing with spliced leader RNA., Molecular and Cellular Biology, 14(7), pp. 4565-4570. https://doi.org/10.1128/MCB.14.7.4565
APA Citation style: Xu, G., Wieland, B., & Bindereif, A. (1994). Trans-spliceosomal U6 RNAs of Crithidia fasciculata and Leptomonas seymouri: deviation from the conserved ACAGAG sequence and potential base pairing with spliced leader RNA.. Molecular and Cellular Biology. 14(7), 4565-4570. https://doi.org/10.1128/MCB.14.7.4565