Journalartikel

Jahr der Veröffentlichung: 1986

Seiten: 265-275

Zeitschrift: Roux's archives of developmental biology

Bandnummer: 195

Heftnummer: 4

ISSN: 0930-035X

DOI Link: https://doi.org/10.1007/BF02438960

Verlag: Springer

Abstract: 

In 32-cell stage embryos ofPatella vulgata one of the macromeres contacts the animal micromeres, and as a result is induced to differentiate into the stem cell of the mesodermal cell line. In this study we show the presence of an extracellular matrix (ECM) between these two interacting cell types. The ECM appears to be formed by the micromeres during the 32-cell stage. Staining experiments with alcian blue and tannic acid indicate that in contains glycoconjugates, possibly in the form of proteoglycans. The characteristics of the ECM were examined further by fluorescein isothiocyanate (FITC)-lectin labelling. Of 17 lectins tested, concanavalin A (ConA), succinyl-ConA, LCH-B (Lens culinaris) and PEA (Pisum sativum) showed a positive labelling of the ECM. These results are in accordance with the electron microscopic data. The appearance of the ECM at this specific stage and place suggests that it might play an important role in the induction of the mesodermal cell line.

Harvard-Zitierstil: Kühtreiber, W., van der Bent, J., Dorresteijn, A., de Graaf, A., van den Biggelaar, J. and van Dongen, C. (1986) The presence of an extracellular matrix between cells involved in the determination of the mesoderm bands in embryos of Patella vulgata (Mollusca, gastropoda), Roux's archives of developmental biology, 195(4), pp. 265-275. https://doi.org/10.1007/BF02438960

APA-Zitierstil: Kühtreiber, W., van der Bent, J., Dorresteijn, A., de Graaf, A., van den Biggelaar, J., & van Dongen, C. (1986). The presence of an extracellular matrix between cells involved in the determination of the mesoderm bands in embryos of Patella vulgata (Mollusca, gastropoda). Roux's archives of developmental biology. 195(4), 265-275. https://doi.org/10.1007/BF02438960