A new U6 small nuclear ribonucleoprotein-specific protein conserved between cis- and trans-splicing systems. - Research portal of Justus Liebig University Giessen:

Journal article

A new U6 small nuclear ribonucleoprotein-specific protein conserved between cis- and trans-splicing systems.

Authors list: Gröning, K; Palfi, Z; Gupta, S; Cross, M; Wolff, T; Bindereif, A

Publication year: 1991

Pages: 2026-2034

Journal: Molecular and Cellular Biology

Volume number: 11

Issue number: 4

ISSN: 0270-7306

eISSN: 1098-5549

URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC359889/

Publisher: Taylor and Francis Group

Abstract:
Spliceosomal U6 small nuclear RNA (snRNA) plays a central role in the pre-mRNA splicing mechanism and is highly conserved throughout evolution. Previously, a sequence element essential for both capping and cytoplasmic-nuclear transport of U6 snRNA was mapped in the 5'-terminal domain of U6 snRNA. We have identified a protein in cytoplasmic extracts of mammalian and Trypanosoma brucei cells that binds specifically to this U6 snRNA element. Competition studies with mutant and heterologous RNAs demonstrated the conserved binding specificity of the mammalian and trypanosomal proteins. The in vitro capping analysis of mutant U6 snRNAs indicated that protein binding is required but not sufficient for capping of U6 snRNA by a gamma-monomethyl phosphate. Through RNA affinity purification of mammalian small nuclear ribonucleoproteins (snRNPs), we detected this protein also in nuclear extract as a new specific component of the U6 snRNP but surprisingly not of the U4/U6 or the U4/U5/U6 multi-snRNP. These results suggest that the U6-specific protein is involved in U6 snRNA maturation and transport and may therefore be functionally related to the Sm proteins of the other spliceosomal snRNPs.

Citation Styles

Harvard Citation style: Gröning, K., Palfi, Z., Gupta, S., Cross, M., Wolff, T. and Bindereif, A. (1991) A new U6 small nuclear ribonucleoprotein-specific protein conserved between cis- and trans-splicing systems., Molecular and Cellular Biology, 11(4), pp. 2026-2034. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC359889/

APA Citation style: Gröning, K., Palfi, Z., Gupta, S., Cross, M., Wolff, T., & Bindereif, A. (1991). A new U6 small nuclear ribonucleoprotein-specific protein conserved between cis- and trans-splicing systems.. Molecular and Cellular Biology. 11(4), 2026-2034. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC359889/