Journal article

Publication year: 2007

Pages: 145-150

Journal: Avian Pathology

Volume number: 36

Issue number: 2

ISSN: 0307-9457

eISSN: 1465-3338

DOI Link: https://doi.org/10.1080/03079450701213347

Publisher: Taylor and Francis Group

Abstract: 
Mycoplasmas are commensals and pathogens of different avian species, especially poultry and passeriforms. The role of mycoplasmas in raptors has not yet been completely determined, and especially not the possibility of vertical transmission. Therefore 424 raptor eggs were examined for the occurrence of mycoplasmas using culture, and 155 of these eggs with a Mycoplasma genus-specific polymerase chain reaction (PCR) assay. This PCR was tested for its sensitivity and specificity, especially for use in a bird population of unknown mycoplasma status (prevalence and species). The size of the amplified PCR product was large (1013 base pairs) to enable use of the product for species differentiation by sequencing. Culture and PCR yielded only one positive result, in an egg of a Northern Goshawk (Accipiter gentilis). The isolate was identified as Mycoplasma lipofaciens using an immunobinding assay, as well as by sequencing part of its 16S rRNA gene.

Harvard Citation style: Lierz, M., Hagen, N., Harcourt-Brown, N., Hernandez-Divers, S., Lüschow, D. and Hafez, H. (2007) Prevalence of mycoplasmas in eggs from birds of prey using culture and a genus-specific mycoplasma polymerase chain reaction, Avian Pathology, 36(2), pp. 145-150. https://doi.org/10.1080/03079450701213347

APA Citation style: Lierz, M., Hagen, N., Harcourt-Brown, N., Hernandez-Divers, S., Lüschow, D., & Hafez, H. (2007). Prevalence of mycoplasmas in eggs from birds of prey using culture and a genus-specific mycoplasma polymerase chain reaction. Avian Pathology. 36(2), 145-150. https://doi.org/10.1080/03079450701213347