Journalartikel
Autorenliste: Cross, M; Wieland, B; Palfi, Z; Günzl, A; Röthlisberger, U; Lahm, HW; Bindereif, A
Jahr der Veröffentlichung: 1993
Seiten: 1239-1248
Zeitschrift: The EMBO Journal
Bandnummer: 12
Heftnummer: 3
ISSN: 0261-4189
eISSN: 1460-2075
DOI Link: https://doi.org/10.1002/j.1460-2075.1993.tb05765.x
Verlag: EMBO Press
Abstract:
Through immunoscreening we have isolated a cDNA encoding the trans-spliceosomal U2 snRNP-specific 40 kDa protein of Trypanosoma brucei. The protein has a predicted molecular weight of 36.6 kDa and shows 31% amino acid identity with the human U2 snRNP A' protein of 28.4 kDa. The homology between the trypanosome and human protein sequences is restricted to the N-terminal half where they share a series of six leucine repeat motifs. Sequence alignment revealed three 40K-specific regions: a C-terminal extension and two insertions, one of which makes up a seventh leucine repeat. Bacterially expressed 40K protein efficiently bound RNA by itself in a nonspecific manner; this general RNA binding activity was located to a region in the C-terminal half overlapping with the leucine repeat domain. U2 RNA-specific interaction required the presence of other trypanosome proteins and depended upon the loop IV sequence of U2 RNA. Deletion analysis of the 40K protein demonstrated the leucine repeats, including the 40K-specific, seventh repeat, to be essential for specific U2 RNP assembly, most likely through their role as an interface for protein-protein interaction.
Zitierstile
Harvard-Zitierstil: Cross, M., Wieland, B., Palfi, Z., Günzl, A., Röthlisberger, U., Lahm, H., et al. (1993) The trans-spliceosomal U2 snRNP protein 40K of Trypanosoma brucei: cloning and analysis of functional domains reveals homology to a mammalian snRNP protein., The EMBO Journal, 12(3), pp. 1239-1248. https://doi.org/10.1002/j.1460-2075.1993.tb05765.x
APA-Zitierstil: Cross, M., Wieland, B., Palfi, Z., Günzl, A., Röthlisberger, U., Lahm, H., & Bindereif, A. (1993). The trans-spliceosomal U2 snRNP protein 40K of Trypanosoma brucei: cloning and analysis of functional domains reveals homology to a mammalian snRNP protein.. The EMBO Journal. 12(3), 1239-1248. https://doi.org/10.1002/j.1460-2075.1993.tb05765.x