Journalartikel

Jahr der Veröffentlichung: 1999

Seiten: 76-83

Zeitschrift: Clinical & Experimental Immunology

Bandnummer: 117

Heftnummer: 1

ISSN: 0009-9104

DOI Link: https://doi.org/10.1046/j.1365-2249.1999.00936.x

Verlag: Oxford University Press

Abstract: 
HIV-1 can be neutralized by soluble factors produced and secreted by activated CD8(+) T cells. Production of such anti-viral CD8 factors (including chemokines) can be induced with IL-2 or phytohaemagglutinin (PHA). In addition to PHA or IL-2, we have co-stimulated CD8(+) T cells with PHA/IL-2 and a mixture of thymic peptides (TP) of molecular weights below 10 kD. For the activation, CD8(+) T cells were purified from peripheral blood mononuclear cells of HIV-1(-) individuals and any resultant anti-viral activity was monitored using an HIV-1 neutralization assay. Using HIV-1 isolates highly resistant to chemokine inhibition we detected significantly higher levels of HIV-1 neutralizing activity in CD8(+) T cell culture supernatants which had been co-activated with TP. When the TP-induced anti-viral activity was monitored, neutralization of both non-syncytia-inducing (NSI) and syncytia-inducing (SI) patient isolates was enhanced by 38% (NSI, PHA +/- TP), 66% (SI, PHA +/- TP), 28% (NSI, IL-2 +/- TP), and 57% (SI, IL-2 +/- TP) compared with the anti-viral activity present in supernatants from CD8(+) T cell cultures stimulated only with PHA or IL-2. Peptide sequence analysis of purified TP showed that the TP mixture predominantly contains peptides with homology to human histone and collagen sequences. Our data demonstrate that CD8(+) T cells are additionally activated by a mixture of TP. In this way, the production of HIV-1 neutralizing CD8 factors can be enhanced.

Harvard-Zitierstil: Müller, H., Mayer, G., Behnke, B., Heimüller, E., Hamscher, G., Immler, D., et al. (1999) Enhancing of anti-viral activity against HIV-1 by stimulation of CD8(+) T cells with thymic peptides, Clinical & Experimental Immunology, 117(1), pp. 76-83. https://doi.org/10.1046/j.1365-2249.1999.00936.x

APA-Zitierstil: Müller, H., Mayer, G., Behnke, B., Heimüller, E., Hamscher, G., Immler, D., Siethoff, C., Meyer, H., & Schreiber, M. (1999). Enhancing of anti-viral activity against HIV-1 by stimulation of CD8(+) T cells with thymic peptides. Clinical & Experimental Immunology. 117(1), 76-83. https://doi.org/10.1046/j.1365-2249.1999.00936.x