Assessing bioactive tetracycline in soil samples - Application of a German-Standard-Bioassay DIN 58940 part 1-3 - Research portal of Justus Liebig University Giessen:

Journal article

Assessing bioactive tetracycline in soil samples - Application of a German-Standard-Bioassay DIN 58940 part 1-3

Authors list: Quack, B; Fründ, HC; Höper, H; Hamscher, G

Publication year: 2005

Pages: 107-111

Journal: Journal of Soils and Sediments

Volume number: 5

Issue number: 2

ISSN: 1439-0108

DOI Link: https://doi.org/10.1065/jss2004.10.122

Publisher: Springer

Abstract:
Background. Tetracycline (TC) is one of the most common antibiotics in animal husbandry. Concentrations of up to 200 mu g TC kg(-1) have been found in soils, which have repeatedly received pig manure, and it can be assumed that there is an accumulation in soil.Methods. The established methods for chemical analysis of extractable TC in soil employ HPLC-UV or HPLC-MS-MS. These methods are quite expensive and require well-trained laboratory personnel and sophisticated high-technology equipment. For this reason, a German Standard Bioassay (DIN 58940 Part 1-3) was adjusted and validated in order to establish an alternative low-priced method for monitoring TC contents in soil samples. The tests were conducted with two soils (sand and loamy sand) spiked with TC at 6 concentrations. With this bioassay, TC in soils was analyzed and a simple quantification procedure was established. It will be demonstrated that sand and loamy sand soil properties have an influence on the recoveries of extractable, bioactive TC.Results. In the loamy sand soil, less bioactive TC was extracted (15-25%) than in the sandy soil (30-55%), when tested against the aqueous TC-solution (100%) as a control. These results were confirmed by HPLC-MS-MS analyses. The bioassay was successfully employed in a degradation study in a greenhouse pot experiment.Recommendation. It could prove to be expedient to use this bioassay in the assessment of other antibiotics as well.

Authors/Editors

- Uni.-Prof. Dr. Gerd Hamscher

Citation Styles

Harvard Citation style: Quack, B., Fründ, H., Höper, H. and Hamscher, G. (2005) Assessing bioactive tetracycline in soil samples - Application of a German-Standard-Bioassay DIN 58940 part 1-3, Journal of Soils and Sediments, 5(2), pp. 107-111. https://doi.org/10.1065/jss2004.10.122

APA Citation style: Quack, B., Fründ, H., Höper, H., & Hamscher, G. (2005). Assessing bioactive tetracycline in soil samples - Application of a German-Standard-Bioassay DIN 58940 part 1-3. Journal of Soils and Sediments. 5(2), 107-111. https://doi.org/10.1065/jss2004.10.122