Journal article
Authors list: Schnappauf, F; Hake, SB; Carvajal, MMC; Bontron, S; Lisowska-Grospierre, B; Steimle, V
Publication year: 2003
Pages: 2337-2347
Journal: European Journal of Immunology
Volume number: 33
Issue number: 8
ISSN: 0014-2980
DOI Link: https://doi.org/10.1002/eji.200323490
Publisher: Wiley
Abstract:
Major histocompatibility complex (MHC) class II molecules play an essential role for the cellular immune response by presenting peptide antigens to CD4(+) T cells. MHC class II molecules and genes show a highly complex expression pattern, which is orchestrated through a master regulatory factor, called CIITA (class II transactivator). CIITA controls MHC class II expression not only qualitatively, but also quantitatively, and has therefore a direct influence on the CD4 T cell-dependent immune response. CIITA is itself tightly regulated not only on the transcriptional level, but as we show here also on the protein level. CIITA is subjected to a very rapid protein turnover and shows a half-life of about 30 min. Inhibition of degradation by proteasome inhibitors and the identification of ubiquitylated CIITA intermediates indicate that the degradation of CIITA is mediated by the ubiquitin-proteasome system. We identified two regions mediating degradation within the N-terminal domain of CIITA. N-terminal fusions or deletions stabilized CIITA, indicating that the N termini contribute to degradation. Several non-functional CIITA mutants are partially stabilized, but we provide evidence that transcriptional activity of CIITA is not directly linked to degradation.
Citation Styles
Harvard Citation style: Schnappauf, F., Hake, S., Carvajal, M., Bontron, S., Lisowska-Grospierre, B. and Steimle, V. (2003) N-terminal destruction signals lead to rapid degradation of the major histocompatibility complex class II transactivator CIITA, European Journal of Immunology, 33(8), pp. 2337-2347. https://doi.org/10.1002/eji.200323490
APA Citation style: Schnappauf, F., Hake, S., Carvajal, M., Bontron, S., Lisowska-Grospierre, B., & Steimle, V. (2003). N-terminal destruction signals lead to rapid degradation of the major histocompatibility complex class II transactivator CIITA. European Journal of Immunology. 33(8), 2337-2347. https://doi.org/10.1002/eji.200323490