Journalartikel

Jahr der Veröffentlichung: 2016

Seiten: 31313-

Zeitschrift: Scientific Reports

Bandnummer: 6

ISSN: 2045-2322

eISSN: 2045-2322

Open Access Status: Gold

DOI Link: https://doi.org/10.1038/srep31313

Verlag: Nature Research

Abstract: 
Circular RNAs (circRNAs) constitute a new class of noncoding RNAs in higher eukaryotes generated from pre-mRNAs by alternative splicing. Here we investigated in mammalian cells the association of circRNAs with proteins. Using glycerol gradient centrifugation, we characterized in cell lysates circRNA-protein complexes (circRNPs) of distinct sizes. By polysome-gradient fractionation we found no evidence for efficient translation of a set of abundant circRNAs in HeLa cells. To identify circRNPs with a specific protein component, we focused on IMP3 (IGF2BP3, insulin-like growth factor 2 binding protein 3), a known tumor marker and RNA-binding protein. Combining RNA-seq analysis of IMP3-coimmunoprecipitated RNA and filtering for circular-junction reads identified a set of IMP3-associated circRNAs, which were validated and characterized. In sum, our data suggest that specific circRNP families exist defined by a common protein component. In addition, this provides a general approach to identify circRNPs with a given protein component.

Harvard-Zitierstil: Schneider, T., Hung, L., Schreiner, S., Starke, S., Eckhof, H., Rossbach, O., et al. (2016) CircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPs, Scientific Reports, 6, p. 31313. https://doi.org/10.1038/srep31313

APA-Zitierstil: Schneider, T., Hung, L., Schreiner, S., Starke, S., Eckhof, H., Rossbach, O., Reich, S., Medenbach, J., & Bindereif, A. (2016). CircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPs. Scientific Reports. 6, 31313. https://doi.org/10.1038/srep31313