Journal article

Publication year: 1989

Pages: 396-402

Journal: Berichte der Bunsengesellschaft für physikalische Chemie

Volume number: 93

Issue number: 3

ISSN: 0005-9021

DOI Link: https://doi.org/10.1002/bbpc.19890930335

Publisher: Wiley

Abstract: 

Since the development of laser microprobe mass analysis, a large number of investigations have shown the versatility of the technique for biological and medical applications. The main advantages are high sensitivity and a spatial resolution in the μm‐range. Several approaches were used to test the possibility for a quantitative analysis, using internal standards. So far absolute quantification is in general not possible. Nearly all laser microanalysis investigations concerned the spatial distributions of elements (mainly metal ions) in a biological matrix. In a few cases the local organic composition was mapped by “fingerprinting”. Only in a small number of investigations could molecular constituents in the sampled microarea be observed and identified directly out of their native surroundings. Laser desorption/ionization (LDI) as an ionization method for mass spectrometric analysis of bioorganic substances on the other hand has proven to be a useful technique of its own. For ultraviolet‐LDI pioneering work has been done towards understanding of the processes of energy deposition and ion formation. As a result of this a matrix technique was developed, which now is able to generate intact molecular ions of proteins up to the 100000 dalton mass range and above.

Harvard Citation style: Spengler, B., Karas, M., Bahr, U. and Hillenkamp, F. (1989) Laser mass analysis in biology, Berichte der Bunsengesellschaft für physikalische Chemie, 93(3), pp. 396-402. https://doi.org/10.1002/bbpc.19890930335

APA Citation style: Spengler, B., Karas, M., Bahr, U., & Hillenkamp, F. (1989). Laser mass analysis in biology. Berichte der Bunsengesellschaft für physikalische Chemie. 93(3), 396-402. https://doi.org/10.1002/bbpc.19890930335