Journalartikel
Autorenliste: Heiner, M; Hui, JY; Schreiner, S; Hung, LH; Bindereif, A
Jahr der Veröffentlichung: 2010
Seiten: 56-64
Zeitschrift: RNA Biology
Bandnummer: 7
Heftnummer: 1
ISSN: 1547-6286
eISSN: 1555-8584
Open Access Status: Bronze
DOI Link: https://doi.org/10.4161/rna.7.1.10402
Verlag: Taylor and Francis Group
Abstract:
Heterogeneous nuclear ribonucleoprotein (hnRNP) L can regulate alternative mRNA splicing in diverse ways, binding to exonic or intronic sites and acting as either an activator or repressor. To investigate the mechanistic basis of hnRNP L-regulated alternative splicing, we focus here on two specific cases of hnRNP L-dependent splice site recognition. First, in the case of TJP1 our microarray data had suggested that exon 20 inclusion is regulated by hnRNP L as a repressor. Here we demonstrate by mutational analysis that exon skipping is mediated by a short silencer sequence consisting of three hnRNP L high-score binding motifs located upstream of the 3' splice site of the regulated exon. UV crosslinking and immunoprecipitation experiments showed that hnRNP L binding interferes with 3' splice site recognition by U2AF65. Second, SLC2A2 contains a CA-repeat sequence close to the 5' splice site of the regulated exon 4. Using psoralen crosslinking, we demonstrate that hnRNP L represses splicing by preventing 5' splice site recognition of the U1 snRNP. In sum, our data provide new insights into the mechanisms of how hnRNP L-bound to intronic sites-regulates exon recognition.
Zitierstile
Harvard-Zitierstil: Heiner, M., Hui, J., Schreiner, S., Hung, L. and Bindereif, A. (2010) HnRNP L-mediated regulation of mammalian alternative splicing by interference with splice site recognition, RNA Biology, 7(1), pp. 56-64. https://doi.org/10.4161/rna.7.1.10402
APA-Zitierstil: Heiner, M., Hui, J., Schreiner, S., Hung, L., & Bindereif, A. (2010). HnRNP L-mediated regulation of mammalian alternative splicing by interference with splice site recognition. RNA Biology. 7(1), 56-64. https://doi.org/10.4161/rna.7.1.10402