Sammelbandbeitrag

Erschienen in: Circular RNAs : Methods and Protocols

Herausgeberliste: Dieterich, C; Papantonis, A

Jahr der Veröffentlichung: 2018

Seiten: 119-133

ISSN: 1064-3745

ISBN: 978-1-4939-7561-7

eISBN: 978-1-4939-7562-4

DOI Link: https://doi.org/10.1007/978-1-4939-7562-4_10

Serientitel: Methods in Molecular Biology

Serienzählung: 1724

Abstract: 
Northern blotting enables the specific detection and characterization of RNA molecules. Recently, circular RNAs (circRNAs) were described as a new class of cell type-specific noncoding RNAs. With the discovery of many novel circRNAs on the basis of high-throughput sequencing and bioinformatics, a solid biochemical approach is required to directly detect and validate specific circRNA species. Here we give a detailed overview of how different Northern blot methods can be employed to validate specific circRNAs. Different Northern gel and detection systems are introduced, in combination with additional tools for circRNA characterization, such as RNase R and RNase H treatments.

Harvard-Zitierstil: Schneider, T., Schreiner, S., Preußer, C., Bindereif, A. and Rossbach, O. (2018) Northern Blot Analysis of Circular RNAs, in Dieterich, C. and Papantonis, A. (eds.) Circular RNAs : Methods and Protocols. New York: Humana Press, pp. 119-133. https://doi.org/10.1007/978-1-4939-7562-4_10

APA-Zitierstil: Schneider, T., Schreiner, S., Preußer, C., Bindereif, A., & Rossbach, O. (2018). Northern Blot Analysis of Circular RNAs. In Dieterich, C., & Papantonis, A. (Eds.), Circular RNAs : Methods and Protocols (pp. 119-133). Humana Press. https://doi.org/10.1007/978-1-4939-7562-4_10