Journal article

Publication year: 2003

Pages: 33-37

Journal: Nature Structural Biology

Volume number: 10

Issue number: 1

ISSN: 1072-8368

DOI Link: https://doi.org/10.1038/nsb875

Publisher: Nature America

Abstract: 
In the human genome, dinucleotide repeats are common sequence elements of unknown functional significance. Here we demonstrate that CA repeats in intron 13 of the human endothelial nitric oxide synthase (eNOS) gene function as an unusual intronic splicing enhancer, whose activity depends on the CA repeat number. We identify the 65 kDa heterogenous nuclear ribonucleoprotein (hnRNP) L as the major factor that binds specifically and in a length-dependent manner to the CA-repeat enhancer. In addition, we show that hnRNP L functions as a specific activator of eNOS splicing, providing the first evidence for a role of hnRNP L in the regulation of mRNA splicing. We hypothesize that hnRNP L may be involved in the regulation of many other genes containing CA repeats or A/C-rich enhancers.

Harvard Citation style: Hui, J., Stangl, K., Lane, W. and Bindereif, A. (2003) HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats, Nature Structural Biology, 10(1), pp. 33-37. https://doi.org/10.1038/nsb875

APA Citation style: Hui, J., Stangl, K., Lane, W., & Bindereif, A. (2003). HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats. Nature Structural Biology. 10(1), 33-37. https://doi.org/10.1038/nsb875