The histone variant H2A.Bbd is enriched at sites of DNA synthesis - Research portal of Justus Liebig University Giessen:

Journal article

The histone variant H2A.Bbd is enriched at sites of DNA synthesis

Authors list: Sansoni, V; Casas-Delucchi, CS; Rajan, M; Schmidt, A; Bönisch, C; Thomae, AW; Staege, MS; Hake, SB; Cardoso, MC; Imhof, A

Publication year: 2014

Pages: 6405-6420

Journal: Nucleic Acids Research

Volume number: 42

Issue number: 10

ISSN: 0305-1048

DOI Link: https://doi.org/10.1093/nar/gku303

Publisher: Oxford University Press

Abstract:
Histone variants play an important role in shaping the mammalian epigenome and their aberrant expression is frequently observed in several types of cancer. However, the mechanisms that mediate their function and the composition of the variant-containing chromatin are still largely unknown. A proteomic interrogation of chromatin containing the different H2A variants macroH2A.1.2, H2A.Bbd and H2A revealed a strikingly different protein composition. Gene ontology analysis reveals a strong enrichment of splicing factors as well as components of the mammalian replisome in H2A.Bbd-containing chromatin. We find H2A.Bbd localizing transiently to sites of DNA synthesis during S-phase and during DNA repair. Cells that express H2A.Bbd have a shortened S-phase and are more susceptible to DNA damage, two phenotypes that are also observed in human Hodgkin's lymphoma cells that aberrantly express this variant. Based on our experiments we conclude that H2A.Bbd is targeted to newly synthesized DNA during replication and DNA repair. The transient incorporation of H2A.Bbd may be due to the intrinsic instability of nucleosomes carrying this variant or a faster chromatin loading. This potentially leads to a disturbance of the existing chromatin structure, which may have effects on cell cycle regulation and DNA damage sensitivity.

Authors/Editors

- Uni.-Prof. Dr. Sandra B. Hake

Citation Styles

Harvard Citation style: Sansoni, V., Casas-Delucchi, C., Rajan, M., Schmidt, A., Bönisch, C., Thomae, A., et al. (2014) The histone variant H2A.Bbd is enriched at sites of DNA synthesis, Nucleic Acids Research, 42(10), pp. 6405-6420. https://doi.org/10.1093/nar/gku303

APA Citation style: Sansoni, V., Casas-Delucchi, C., Rajan, M., Schmidt, A., Bönisch, C., Thomae, A., Staege, M., Hake, S., Cardoso, M., & Imhof, A. (2014). The histone variant H2A.Bbd is enriched at sites of DNA synthesis. Nucleic Acids Research. 42(10), 6405-6420. https://doi.org/10.1093/nar/gku303