Journalartikel

Jahr der Veröffentlichung: 2005

Seiten: 1988-1998

Zeitschrift: The EMBO Journal

Bandnummer: 24

Heftnummer: 11

ISSN: 0261-4189

eISSN: 1460-2075

Open Access Status: Green

DOI Link: https://doi.org/10.1038/sj.emboj.7600677

Verlag: EMBO Press

Abstract: 
We have recently identified an intronic polymorphic CA-repeat region in the human endothelial nitric oxide synthase ( eNOS) gene as an important determinant of the splicing efficiency, requiring specific binding of hnRNP L. Here, we analyzed the position requirements of this CA-repeat element, which revealed its potential role in alternative splicing. In addition, we defined the RNA binding specificity of hnRNP L by SELEX: not only regular CA repeats are recognized with high affinity but also certain CA-rich clusters. Therefore, we have systematically searched the human genome databases for CA-repeat and CA-rich elements associated with alternative 5' splice sites (5'ss), followed by minigene transfection assays. Surprisingly, in several specific human genes that we tested, intronic CA RNA elements could function either as splicing enhancers or silencers, depending on their proximity to the alternative 5'ss. HnRNP L was detected specifically bound to these diverse CA elements. These data demonstrated that intronic CA sequences constitute novel and widespread regulatory elements of alternative splicing.

Harvard-Zitierstil: Hui, J., Hung, L., Heiner, M., Schreiner, S., Neumüller, N., Reither, G., et al. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing, The EMBO Journal, 24(11), pp. 1988-1998. https://doi.org/10.1038/sj.emboj.7600677

APA-Zitierstil: Hui, J., Hung, L., Heiner, M., Schreiner, S., Neumüller, N., Reither, G., Haas, S., & Bindereif, A. (2005). Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. The EMBO Journal. 24(11), 1988-1998. https://doi.org/10.1038/sj.emboj.7600677