Journalartikel

Jahr der Veröffentlichung: 1994

Seiten: 30620-30625

Zeitschrift: Journal of Biological Chemistry

Bandnummer: 269

Heftnummer: 48

ISSN: 0021-9258

eISSN: 1083-351X

URL: https://www.ncbi.nlm.nih.gov/pubmed/7982982

Verlag: Elsevier

Abstract: 
A spliced leader-associated (SLA) RNA recently identified in Trypanosoma brucei has been proposed as a U5 RNA analog in trans-splicing, based on in vivo psoralen cross-linking to the spliced leader (SL) RNA and a short stretch of homology to a conserved U5 RNA sequence. Here we show that the SLA RNA is in the form of an RNP containing common proteins that also occur in the known trans-spliceosomal snRNPs of T. brucei, the SL, U2, and U4/U6 snRNPs. Therefore the SLA RNP is a member of a larger family of related RNPs. The immunoprecipitation of the SLA RNA by antibodies against common proteins does not require an association between the SLA RNA and the SL RNP. To identify sequences of potential functional importance and to establish a secondary structure model, we have sequenced the SLA RNA and cloned its gene from a related trypanosomatid, Leptomonas seymouri. A sequence comparison between the T. brucei and L. seymouri SLA RNAs reveals that the U5-homologous sequence element is not conserved.

Harvard-Zitierstil: Palfi, Z., Xu, G. and Bindereif, A. (1994) Spliced leader-associated RNA of trypanosomes. Sequence conservation and association with protein components common to trans-spliceosomal ribonucleoproteins., Journal of Biological Chemistry, 269(48), pp. 30620-30625. https://www.ncbi.nlm.nih.gov/pubmed/7982982

APA-Zitierstil: Palfi, Z., Xu, G., & Bindereif, A. (1994). Spliced leader-associated RNA of trypanosomes. Sequence conservation and association with protein components common to trans-spliceosomal ribonucleoproteins.. Journal of Biological Chemistry. 269(48), 30620-30625. https://www.ncbi.nlm.nih.gov/pubmed/7982982