Frequent promoter methylation of tumor-related genes in sporadic and Men2-associated pheochromocytomas - Research portal of Justus Liebig University Giessen:

Journal article

Frequent promoter methylation of tumor-related genes in sporadic and Men2-associated pheochromocytomas

Authors list: Dammann, R; Schagdarsurengin, U; Seidel, C; Trümpler, C; Hoang-Vu, C; Gimm, O; Dralle, H; Pfeifer, GP; Brauckhoff, M

Publication year: 2005

Pages: 1-7

Journal: Experimental and Clinical Endocrinology & Diabetes

Volume number: 113

Issue number: 1

ISSN: 0947-7349

eISSN: 1439-3646

DOI Link: https://doi.org/10.1055/s-2004-830522

Publisher: Thieme Publishing

Abstract:
Hypermethylation of CpG island promoters is associated with transcriptional inactivation of tumor suppressor genes in neoplasia. Inactivation of p16 and Pten was related to the development of pheochromocytomas. In this report, we investigated the methylation status of the p16(INK4a) cell cycle inhibitor gene and other prominent tumor-related genes (PTEN, RASSF1 A, CDH1, MSH2, MLH1, VHL, and TIMP3) in sporadic and multiple endocrine neoplasia type 2 (MEN2) pheochromocytomas by methylation-specific PCR. Hypermethylation was detected in 48% of pheochromocytomas for RASSF1 A, 24% for p16, 36% for MSH2, 16% for CDH1, and 8% for PTEN. No VHL, MLH1, and TIMP3 methylation was observed. Interestingly, the frequency of p16 inactivation in familial tumors was higher (5 out of 12, 42%) than in sporadic tumors (1 out of 13, 8%; p = 0.047) and RASSF1 A inactivation was more common in the hereditary tumors (58%) compared to the sporadic tumors (38%). Combined methylation of RASSF1 A and p 16 was found only in MEN2-related pheochromocytomas. Thus, a subset of hereditary pheochromocytomas displays preferential methylation of p 16 and RASSF1 A.

Authors/Editors

- Uni.-Prof. Dr. Reinhard Heinrich Dammann

Citation Styles

Harvard Citation style: Dammann, R., Schagdarsurengin, U., Seidel, C., Trümpler, C., Hoang-Vu, C., Gimm, O., et al. (2005) Frequent promoter methylation of tumor-related genes in sporadic and Men2-associated pheochromocytomas, Experimental and Clinical Endocrinology & Diabetes, 113(1), pp. 1-7. https://doi.org/10.1055/s-2004-830522

APA Citation style: Dammann, R., Schagdarsurengin, U., Seidel, C., Trümpler, C., Hoang-Vu, C., Gimm, O., Dralle, H., Pfeifer, G., & Brauckhoff, M. (2005). Frequent promoter methylation of tumor-related genes in sporadic and Men2-associated pheochromocytomas. Experimental and Clinical Endocrinology & Diabetes. 113(1), 1-7. https://doi.org/10.1055/s-2004-830522