Analysis of small nuclear ribonucleoproteins (RNPs) in Trypanosoma brucei: structural organization and protein components of the spliced leader RNP. - Forschungsportal der Justus-Liebig-Universität Gießen:

Journalartikel

Analysis of small nuclear ribonucleoproteins (RNPs) in Trypanosoma brucei: structural organization and protein components of the spliced leader RNP.

Autorenliste: Cross, M; Günzl, A; Palfi, Z; Bindereif, A

Jahr der Veröffentlichung: 1991

Seiten: 5516-5526

Zeitschrift: Molecular and Cellular Biology

Bandnummer: 11

Heftnummer: 11

ISSN: 0270-7306

eISSN: 1098-5549

DOI Link: https://doi.org/10.1128/MCB.11.11.5516

Verlag: Taylor and Francis Group

Abstract:
trans splicing in Trypanosoma brucei involves the ligation of the 40-nucleotide spliced leader (SL) to each of the exons of large, polycistronic pre-mRNAs and requires the function of small nuclear ribonucleoproteins (snRNPs). We have identified and characterized snRNP complexes of SL, U2, U4, and U6 RNAs in T. brucei extracts by a combination of glycerol gradient sedimentation, CsCl density centrifugation, and anti-m3G immunoprecipitation. Both the SL RNP and the U4/U6 snRNP contain salt-stable cores; the U2 snRNP, in contrast to other eucaryotic snRNPs, is not stable under stringent ionic conditions. Two distinct complexes of U6 RNA were found, a U6 snRNP and a U4/U6 snRNP. The structure of the SL RNP was analyzed in detail by oligonucleotide-directed RNase H protection and by in vitro reconstitution. Our results indicate that the 3' half of SL RNA constitutes the core protein-binding domain and that protein components of the SL RNP also bind to the U2 and U4 RNAs. Using antisense RNA affinity chromatography, we identified a set of low-molecular-mass proteins (14.8, 14, 12.5, and 10 kDa) as components of the core SL RNP.

Zitierstile

Harvard-Zitierstil: Cross, M., Günzl, A., Palfi, Z. and Bindereif, A. (1991) Analysis of small nuclear ribonucleoproteins (RNPs) in Trypanosoma brucei: structural organization and protein components of the spliced leader RNP., Molecular and Cellular Biology, 11(11), pp. 5516-5526. https://doi.org/10.1128/MCB.11.11.5516

APA-Zitierstil: Cross, M., Günzl, A., Palfi, Z., & Bindereif, A. (1991). Analysis of small nuclear ribonucleoproteins (RNPs) in Trypanosoma brucei: structural organization and protein components of the spliced leader RNP.. Molecular and Cellular Biology. 11(11), 5516-5526. https://doi.org/10.1128/MCB.11.11.5516