Journalartikel

Jahr der Veröffentlichung: 2018

Seiten: 237-

Zeitschrift: Nature Communications

Bandnummer: 9

Heftnummer: 1

ISSN: 2041-1723

eISSN: 2041-1723

Open Access Status: Gold

DOI Link: https://doi.org/10.1038/s41467-017-02431-1

Verlag: Nature Research

Abstract: 
Impaired or excessive growth of endothelial cells contributes to several diseases. However, the functional involvement of regulatory long non-coding RNAs in these processes is not well defined. Here, we show that the long non-coding antisense transcript of GATA6 (GATA6-AS) interacts with the epigenetic regulator LOXL2 to regulate endothelial gene expression via changes in histone methylation. Using RNA deep sequencing, we find that GATA6-AS is upregulated in endothelial cells during hypoxia. Silencing of GATA6-AS diminishes TGF-beta 2-induced endothelial-mesenchymal transition in vitro and promotes formation of blood vessels in mice. We identify LOXL2, known to remove activating H3K4me3 chromatin marks, as a GATA6-AS-associated protein, and reveal a set of angiogenesis-related genes that are inversely regulated by LOXL2 and GATA6-AS silencing. As GATA6-AS silencing reduces H3K4me3 methylation of two of these genes, periostin and cyclooxygenase-2, we conclude that GATA6-AS acts as negative regulator of nuclear LOXL2 function.

Harvard-Zitierstil: Neumann, P., Jae, N., Knau, A., Glaser, S., Fouani, Y., Rossbach, O., et al. (2018) The lncRNA GATA6-AS epigenetically regulates endothelial gene expression via interaction with LOXL2, Nature Communications, 9(1), p. 237. https://doi.org/10.1038/s41467-017-02431-1

APA-Zitierstil: Neumann, P., Jae, N., Knau, A., Glaser, S., Fouani, Y., Rossbach, O., Krüger, M., John, D., Bindereif, A., Grote, P., Boon, R., & Dimmeler, S. (2018). The lncRNA GATA6-AS epigenetically regulates endothelial gene expression via interaction with LOXL2. Nature Communications. 9(1), 237. https://doi.org/10.1038/s41467-017-02431-1