Immunological characterization and intracellular localization of trans-spliceosomal small nuclear ribonucleoproteins in Trypanosoma brucei. - Research portal of Justus Liebig University Giessen:

Journal article

Immunological characterization and intracellular localization of trans-spliceosomal small nuclear ribonucleoproteins in Trypanosoma brucei.

Authors list: Palfi, Z; Bindereif, A

Publication year: 1992

Pages: 20159-20163

Journal: Journal of Biological Chemistry

Volume number: 267

Issue number: 28

ISSN: 0021-9258

eISSN: 1083-351X

URL: https://www.ncbi.nlm.nih.gov/pubmed/1400334

Publisher: Elsevier

Abstract:
Polyclonal antibodies were raised against purified protein components of the U2 small nuclear ribonucleoprotein (snRNP) from Trypanosoma brucei. Through immunoblot and immunoprecipitation analyses three antisera were characterized that reacted specifically with U2 snRNP proteins of molecular weights 40,000 (anti-40K) and 16,500 (anti-16.5K), and with each of four proteins of molecular weights 14,000, 12,500, 10,000, and 8,500 (anti-CP). Anti-40K antibodies specifically immunoprecipitated the U2 snRNP from trypanosomal extracts, whereas anti-CP antibodies recognized several snRNPs, including the SL RNP and the U2 and U4/U6 snRNPs; in addition, minor RNAs were detected, suggesting that a family of snRNPs with common or related protein components exists in trypanosomes. None of these antibodies cross-reacted significantly with total mammalian snRNP proteins, indicating that the trypanosomal snRNP proteins are immunologically distinct from their mammalian counterparts. Using immunofluorescence microscopy, the snRNP proteins exhibited a differential cellular distribution. Whereas the 40-kDa protein is localized exclusively in the nucleus, with the nucleolus being excluded, a fraction of the common proteins also resides in the cytoplasm.

Citation Styles

Harvard Citation style: Palfi, Z. and Bindereif, A. (1992) Immunological characterization and intracellular localization of trans-spliceosomal small nuclear ribonucleoproteins in Trypanosoma brucei., Journal of Biological Chemistry, 267(28), pp. 20159-20163. https://www.ncbi.nlm.nih.gov/pubmed/1400334

APA Citation style: Palfi, Z., & Bindereif, A. (1992). Immunological characterization and intracellular localization of trans-spliceosomal small nuclear ribonucleoproteins in Trypanosoma brucei.. Journal of Biological Chemistry. 267(28), 20159-20163. https://www.ncbi.nlm.nih.gov/pubmed/1400334