Journalartikel

Jahr der Veröffentlichung: 2003

Seiten: 931-936

Zeitschrift: RNA

Bandnummer: 9

Heftnummer: 8

ISSN: 1355-8382

eISSN: 1469-9001

Open Access Status: Green

DOI Link: https://doi.org/10.1261/rna.5660803

Verlag: Cold Spring Harbor Laboratory Press

Abstract: 
CA dinucleotide repeat sequences are very common in the human genome. We have recently demonstrated that the polymorphic CA repeats in intron 13 of the human endothelial nitric oxide synthase (eNOS) gene function as an unusual, length-dependent splicing enhancer. The CA repeat enhancer requires for its activity specific binding of hnRNP L. Here we show that in the absence of bound hnRNP L, the pre-mRNA is cleaved directly upstream of the CA repeats. The addition of recombinant hnRNP L restores RNA stability. CA repeats are both necessary and sufficient for this specific cleavage in the 5' adjacent RNA sequence. We conclude that-in addition to its role as a splicing activator-hnRNP L can act in vitro as a sequence-specific RNA protection factor. Based on the wide abundance of CA repetitive sequences in the human genome, this may represent a novel, generally important role of this abundant hnRNP protein.

Harvard-Zitierstil: Hui, J., Reither, G. and Bindereif, A. (2003) Novel functional role of CA repeats and hnRNP L in RNA stability, RNA, 9(8), pp. 931-936. https://doi.org/10.1261/rna.5660803

APA-Zitierstil: Hui, J., Reither, G., & Bindereif, A. (2003). Novel functional role of CA repeats and hnRNP L in RNA stability. RNA. 9(8), 931-936. https://doi.org/10.1261/rna.5660803