Journal article

Publication year: 2011

Pages: 312-320

Journal: Journal of Biotechnology

Volume number: 154

Issue number: 4

ISSN: 0168-1656

eISSN: 1873-4863

DOI Link: https://doi.org/10.1016/j.jbiotec.2011.04.014

Publisher: Elsevier

Abstract: 
The methylotrophic yeast Pichia pastoris (Komagataella phaffii) CBS7435 is the parental strain of commonly used P. pastoris recombinant protein production hosts making it well suited for improving the understanding of associated genomic features. Here, we present a 9.35 Mbp high-quality genome sequence of P. pastoris CBS7435 established by a combination of 454 and Illumina sequencing. An automatic annotation of the genome sequence yielded 5007 protein-coding genes, 124 tRNAs and 29 rRNAs. Moreover, we report the complete DNA sequence of the first mitochondrial genome of a methylotrophic yeast. Fifteen genes encoding proteins, 2 rRNA and 25 tRNA loci were identified on the 35.7 kbp circular, mitochondrial DNA. Furthermore, the architecture of the putative alpha mating factor protein of P. pastoris CBS7435 turned out to be more complex than the corresponding protein of Saccharomyces cerevisiae.

Harvard Citation style: Küberl, A., Schneider, J., Thallinger, G., Anderl, I., Wibberg, D., Hajek, T., et al. (2011) High-quality genome sequence of Pichia pastoris CBS7435, Journal of Biotechnology, 154(4), pp. 312-320. https://doi.org/10.1016/j.jbiotec.2011.04.014

APA Citation style: Küberl, A., Schneider, J., Thallinger, G., Anderl, I., Wibberg, D., Hajek, T., Jaenicke, S., Brinkrolf, K., Goesmann, A., Szczepanowski, R., Pühler, A., Schwab, H., Glieder, A., & Pichler, H. (2011). High-quality genome sequence of Pichia pastoris CBS7435. Journal of Biotechnology. 154(4), 312-320. https://doi.org/10.1016/j.jbiotec.2011.04.014