Journal article

Publication year: 2006

Pages: 9017-9023

Journal: Journal of Agricultural and Food Chemistry

Volume number: 54

Issue number: 24

ISSN: 0021-8561

DOI Link: https://doi.org/10.1021/jf062205i

Publisher: American Chemical Society

Abstract: 
Maximum residue limits (MRLs) have been established by the European Union when tylosin is used therapeutically. They are fixed at 200 mu g/kg for eggs. A highly sensitive and selective quantitative liquid chromatography electrospray ionization tandem mass spectrometry (LC/ESI/MS/MS) method suitable for monitoring tylosin residues in eggs to determine its depletion kinetics was developed and validated. For sample pretreatment all samples were liquid-liquid extracted with citrate buffer (pH 5.0) and acetonitrile. Liquid chromatographic separation was carried out on a reversed phase C18 column employing a 0.5% formic acid/acetonitrile gradient system. The tylosin recovery in eggs at a concentration range from 1.0-400 mu g/kg was > 82% with relative standard deviations between 1.5 and 11.0%. In two experimental studies administrating tylosin via feed (final dosage: 1.5 g/kg) or drinking water (final dosage: 0.5 g/L), no residues above the MRL were found during and after treatment. Moreover, all samples were well below the actual MRL of 200 mu g/kg. Therefore, our residue data suggest that a withholding period for eggs is not required when laying hens are treated with tylosin in recommended dosages via feed or drinking water.

Harvard Citation style: Hamscher, G., Limsuwan, S., Tansakul, N. and Kietzmann, M. (2006) Quantitative analysis of tylosin in eggs by high performance liquid chromatography with electrospray ionization tandem mass spectrometry: Residue depletion kinetics after administration via feed and drinking water in laying hens, Journal of Agricultural and Food Chemistry, 54(24), pp. 9017-9023. https://doi.org/10.1021/jf062205i

APA Citation style: Hamscher, G., Limsuwan, S., Tansakul, N., & Kietzmann, M. (2006). Quantitative analysis of tylosin in eggs by high performance liquid chromatography with electrospray ionization tandem mass spectrometry: Residue depletion kinetics after administration via feed and drinking water in laying hens. Journal of Agricultural and Food Chemistry. 54(24), 9017-9023. https://doi.org/10.1021/jf062205i