Journal article

Publication year: 2008

Pages: 1532-1538

Journal: RNA

Volume number: 14

Issue number: 8

ISSN: 1355-8382

eISSN: 1469-9001

Open access status: Green

DOI Link: https://doi.org/10.1261/rna.1129608

Publisher: Cold Spring Harbor Laboratory Press

Abstract: 
Pre-mRNA splicing proceeds through assembly of the spliceosome complex, catalysis, and recycling. During each cycle the U4/U6.U5 tri-snRNP is disrupted and U4/U6 snRNA base-pairing unwound, releasing separate post-spliceosomal U4, U5, and U6 snRNPs, which have to be recycled to the splicing-competent tri-snRNP. Previous work implicated p110-the human ortholog of the yeast Prp24 protein - and the LSm2-8 proteins of the U6 snRNP in U4/U6 recycling. Here we show in vitro that these proteins bind synergistically to U6 snRNA: Both purified and recombinant LSm2-8 proteins are able to recruit p110 protein to U6 snRNA via interaction with the highly conserved C-terminal region of p110. Furthermore, the presence of a 2',3'-cyclic phosphate enhances the affinity of U6 snRNA for the LSm2-8 proteins and inversely reduces La protein binding, suggesting a direct role of the 3'-terminal phosphorylation in RNP remodeling during U6 biogenesis.

Harvard Citation style: Licht, K., Medenbach, J., Lührmann, R., Kambach, C. and Bindereif, A. (2008) 3 '-cyclic phosphorylation of U6 snRNA leads to recruitment of recycling factor p110 through LSm proteins, RNA, 14(8), pp. 1532-1538. https://doi.org/10.1261/rna.1129608

APA Citation style: Licht, K., Medenbach, J., Lührmann, R., Kambach, C., & Bindereif, A. (2008). 3 '-cyclic phosphorylation of U6 snRNA leads to recruitment of recycling factor p110 through LSm proteins. RNA. 14(8), 1532-1538. https://doi.org/10.1261/rna.1129608