Journalartikel

Jahr der Veröffentlichung: 2008

Seiten: 284-296

Zeitschrift: RNA

Bandnummer: 14

Heftnummer: 2

ISSN: 1355-8382

eISSN: 1469-9001

Open Access Status: Green

DOI Link: https://doi.org/10.1261/rna.725208

Verlag: Cold Spring Harbor Laboratory Press

Abstract: 
Alternative mRNA splicing patterns are determined by the combinatorial control of regulator proteins and their target RNA sequences. We have recently characterized human hnRNP L as a global regulator of alternative splicing, binding to diverse C/A-rich elements. To systematically identify hnRNP L target genes on a genome-wide level, we have combined splice-sensitive microarray analysis and an RNAi-knockdown approach. As a result, we describe 11 target genes of hnRNP L that were validated by RT-PCR and that represent several new modes of hnRNP L-dependent splicing regulation, involving both activator and repressor functions: first, intron retention; second, inclusion or skipping of cassette-type exons; third, suppression of multiple exons; and fourth, alternative poly(A) site selection. In sum, this approach revealed a surprising diversity of splicing-regulatory processes as well as poly(A) site selection in which hnRNP L is involved.

Harvard-Zitierstil: Hung, L., Heiner, M., Hui, J., Schreiner, S., Benes, V. and Bindereif, A. (2008) Diverse roles of hnRNP L in mammalian mRNA processing: A combined microarray and RNAi analysis, RNA, 14(2), pp. 284-296. https://doi.org/10.1261/rna.725208

APA-Zitierstil: Hung, L., Heiner, M., Hui, J., Schreiner, S., Benes, V., & Bindereif, A. (2008). Diverse roles of hnRNP L in mammalian mRNA processing: A combined microarray and RNAi analysis. RNA. 14(2), 284-296. https://doi.org/10.1261/rna.725208