Domain structure of U2 and U4/U6 small nuclear ribonucleoprotein particles from Trypanosoma brucei: identification of trans-spliceosomal specific RNA-protein interactions. - Research portal of Justus Liebig University Giessen:

Journal article

Domain structure of U2 and U4/U6 small nuclear ribonucleoprotein particles from Trypanosoma brucei: identification of trans-spliceosomal specific RNA-protein interactions.

Authors list: Günzl, A; Cross, M; Bindereif, A

Publication year: 1992

Pages: 468-479

Journal: Molecular and Cellular Biology

Volume number: 12

Issue number: 2

ISSN: 0270-7306

eISSN: 1098-5549

URL: https://www.ncbi.nlm.nih.gov/pubmed/1310147

Publisher: Taylor and Francis Group

Abstract:
Maturation of mRNAs in trypanosomes involves trans splicing of the 5' end of the spliced leader RNA and the exons of polycistronic pre-mRNAs, requiring small nuclear ribonucleoproteins (snRNPs) as cofactors. We have mapped protein-binding sites in the U2 and U4/U6 snRNPs by a combination of RNase H protection analysis, native gel electrophoresis, and CsCl density gradient centrifugation. In the U2 snRNP, protein binding occurs primarily in the 3'-terminal domain; through U2 snRNP reconstitution and chemical modification-interference assays, we have identified discrete positions within stem-loop IV of Trypanosoma brucei U2 RNA that are essential for protein binding; significantly, some of these positions differ from the consensus sequence derived from cis-spliceosomal U2 RNAs. In the U4/U6 snRNP, the major protein-binding region is contained within the 3'-terminal half of U4 RNA. In sum, while the overall domain structure of the U2 and U4/U6 snRNPs is conserved between cis- and trans-splicing systems, our data suggest that there are also trans-spliceosomal specific determinants of RNA-protein binding.

Citation Styles

Harvard Citation style: Günzl, A., Cross, M. and Bindereif, A. (1992) Domain structure of U2 and U4/U6 small nuclear ribonucleoprotein particles from Trypanosoma brucei: identification of trans-spliceosomal specific RNA-protein interactions., Molecular and Cellular Biology, 12(2), pp. 468-479. https://www.ncbi.nlm.nih.gov/pubmed/1310147

APA Citation style: Günzl, A., Cross, M., & Bindereif, A. (1992). Domain structure of U2 and U4/U6 small nuclear ribonucleoprotein particles from Trypanosoma brucei: identification of trans-spliceosomal specific RNA-protein interactions.. Molecular and Cellular Biology. 12(2), 468-479. https://www.ncbi.nlm.nih.gov/pubmed/1310147