Journal article

Publication year: 2010

Pages: 142-148

Journal: Journal of Analytical Toxicology

Volume number: 34

Issue number: 3

ISSN: 0146-4760

DOI Link: https://doi.org/10.1093/jat/34.3.142

Publisher: Oxford University Press

Abstract: 

Cytotoxic drugs, previously used only in human medicine, are
increasingly utilized for cancer treatment in veterinary practice. We
developed and validated a liquid chromatography (LC)-electrospray
ionization-tandem mass spectrometry (MS-MS) method to determine
vincristine, vinblastine, cyclophosphamide, and doxorubicin in canine
urine. Sample pretreatment consisted of liquid-liquid extraction, and LC
separation was carried out on an RP C₁₈ column employing a
0.5% formic acid/methanol gradient system. The analytes were detected in
positive ion mode using the MS-MS scan mode. The mean recoveries in six
different urine samples were between 64.2% and 86.9%. Limits of
quantitation were 0.5 µg/L for vincristine and vinblastine, 1 µg/L for
cyclophosphamide, and 5 µg/L for doxorubicin; limits of detection were
approximately 0.25 µg/L for vincristine, vinblastine, and
cyclophosphamide and 0.5 µg/L for doxorubicin. It could be demonstrated
that all investigated drugs are found in urine of dogs undergoing
chemotherapy. In samples from day 1 after chemotherapy, as much as 63
µg/L vincristine, 111 µg/L vinblastine, and 762 µg/L doxorubicin could
be detected. Cyclophosphamide showed only minor concentrations on day 1,
but up to 2583 µg/L could be found directly after chemotherapy. These
initial data show that there might be a potential contamination risk
when administering cytotoxics in veterinary medicine.

Harvard Citation style: Hamscher, G., Mohring, S., Knobloch, A., Eberle, N., Nau, H., Nolte, I., et al. (2010) Determination of Drug Residues in Urine of Dogs Receiving Anti-Cancer Chemotherapy by Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry: Is There An Environmental or Occupational Risk?, Journal of Analytical Toxicology, 34(3), pp. 142-148. https://doi.org/10.1093/jat/34.3.142

APA Citation style: Hamscher, G., Mohring, S., Knobloch, A., Eberle, N., Nau, H., Nolte, I., & Simon, D. (2010). Determination of Drug Residues in Urine of Dogs Receiving Anti-Cancer Chemotherapy by Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry: Is There An Environmental or Occupational Risk?. Journal of Analytical Toxicology. 34(3), 142-148. https://doi.org/10.1093/jat/34.3.142