Journal article

Publication year: 2016

Pages: 221-231

Journal: RNA Biology

Volume number: 13

Issue number: 2

ISSN: 1547-6286

eISSN: 1555-8584

Open access status: Green

DOI Link: https://doi.org/10.1080/15476286.2015.1130208

Publisher: Taylor and Francis Group

Abstract: 
The generation of mature mRNA in the protozoan parasite Trypanosoma brucei requires coupled polyadenylation and trans splicing. In contrast to other eukaryotes, we still know very little on components, mechanisms, and dynamics of the 3 end-processing machinery in trypanosomes. To characterize the catalytic core of the polyadenylation complex in T. brucei, we first identified the poly(A) polymerase [Tb927.7.3780] as the major functional, nuclear-localized enzyme in trypanosomes. In contrast, another poly(A) polymerase, encoded by an intron-containing gene [Tb927.3.3160], localizes mainly in the cytoplasm and appears not to be functional in general 3 end processing of mRNAs. Based on tandem-affinity purification with tagged CPSF160 and mass spectrometry, we identified ten associated components of the trypanosome polyadenylation complex, including homologues to all four CPSF subunits, Fip1, CstF50/64, and Symplekin, as well as two hypothetical proteins. RNAi-mediated knockdown revealed that most of these factors are essential for growth and required for both in vivo polyadenylation and trans splicing, arguing for a general coupling of these two mRNA-processing reactions.

Harvard Citation style: Koch, H., Raabe, M., Urlaub, H., Bindereif, A. and Preusser, C. (2016) The polyadenylation complex of Trypanosoma brucei: Characterization of the functional poly(A) polymerase, RNA Biology, 13(2), pp. 221-231. https://doi.org/10.1080/15476286.2015.1130208

APA Citation style: Koch, H., Raabe, M., Urlaub, H., Bindereif, A., & Preusser, C. (2016). The polyadenylation complex of Trypanosoma brucei: Characterization of the functional poly(A) polymerase. RNA Biology. 13(2), 221-231. https://doi.org/10.1080/15476286.2015.1130208