Journal article

Publication year: 2002

Pages: 233-243

Journal: Molecular and Biochemical Parasitology

Volume number: 121

Issue number: 2

ISSN: 0166-6851

DOI Link: https://doi.org/10.1016/S0166-6851(02)00044-0

Publisher: Elsevier

Abstract: 
Recent studies in the trypanosome system have revealed that in addition to trans splicing of a short spliced leader (SL) exon, there is also cis splicing of internal introns. It has been suggested that cis splicing requires base-pairing of U1 small nuclear RNA (snRNA) and the 5' splice site. We have cloned the gene for U1 snRNA from Trypanosoma brucei and characterized the U1 snRNP. Based on immunoprecipitation and direct mass-spectrometric protein analysis the U1 snRNP contains the common Sm core found also in the known trans-spliceosomal snRNPs U2, U4/U6, and U5. The 5' end of U1 snRNA in the U1 snRNP is accessible for and functional in specific recognition of the 5' splice site of the poly(A) polymerase intron.

Harvard Citation style: Palfi, Z., Lane, W. and Bindereif, A. (2002) Biochemical and functional characterization of the cis-spliceosomal U1 small nuclear RNP from Trypanosoma brucei, Molecular and Biochemical Parasitology, 121(2), pp. 233-243. https://doi.org/10.1016/S0166-6851(02)00044-0

APA Citation style: Palfi, Z., Lane, W., & Bindereif, A. (2002). Biochemical and functional characterization of the cis-spliceosomal U1 small nuclear RNP from Trypanosoma brucei. Molecular and Biochemical Parasitology. 121(2), 233-243. https://doi.org/10.1016/S0166-6851(02)00044-0