Description of Cohnella rhizoplanae sp. nov., isolated from the root surface of soybean (Glycine max) - Research portal of Justus Liebig University Giessen:

Journal article

Description of Cohnella rhizoplanae sp. nov., isolated from the root surface of soybean (Glycine max)

Authors list: Kämpfer, Peter; Glaeser, Stefanie P.; McInroy, John A.; Busse, Hans-Jürgen; Clermont, Dominique; Criscuolo, Alexis

Publication year: 2024

Journal: Antonie van Leeuwenhoek

Volume number: 118

Issue number: 2

ISSN: 0003-6072

eISSN: 1572-9699

Open access status: Hybrid

DOI Link: https://doi.org/10.1007/s10482-024-02051-y

Publisher: Springer

Abstract:
A Gram-staining-positive, aerobic bacterium, designated strain JJ-181 T, was isolated from the root surface of soybean. Based on the 16S rRNA gene sequence similarities, strain JJ-181 T was grouped into the genus Cohnella, most closely related to Cohnella hashimotonis F6_2S_P_1T (98.85%) and C. ginsengisoli GR21-5 T (98.3%). The pairwise average nucleotide identity and digital DNA-DNA hybridisation values of the JJ-181 T genome assembly against publicly available Cohnella type strain genomes were below 84% and 28%, respectively. The fatty acid profile from whole cell hydrolysates, the cell wall diaminoacid, the quinone system, the polar lipid profile, and the polyamine pattern supported the allocation of strain JJ-181 T to the genus Cohnella. In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain JJ-181 T from its closely-related Cohnella species. Functional analysis revealed that strain JJ-181 T has different gene clusters related to swarming motility, chemotaxis ability, and endospore and biofilm formations. The gene content also suggests the ability of strain JJ-181 T to synthesise biotin and riboflavin, as well as indole-3-acetic acid, an important phytohormone for plant growth. Based on polyphasic analyses, strain JJ-181 T can be classified as a new species of the genus Cohnella, for which we propose the name Cohnella rhizoplanae sp. nov., with strain JJ-181 T (= LMG 31678 T = CIP 112018 T = CCM 9031 T = DSM 110650 T) as the type strain.

Authors/Editors

- Uni.-Prof. Dr. Dr. Peter Kämpfer

Citation Styles

Harvard Citation style: Kämpfer, P., Glaeser, S., McInroy, J., Busse, H., Clermont, D. and Criscuolo, A. (2024) Description of Cohnella rhizoplanae sp. nov., isolated from the root surface of soybean (Glycine max), Antonie van Leeuwenhoek, 118(2), Article 41. https://doi.org/10.1007/s10482-024-02051-y

APA Citation style: Kämpfer, P., Glaeser, S., McInroy, J., Busse, H., Clermont, D., & Criscuolo, A. (2024). Description of Cohnella rhizoplanae sp. nov., isolated from the root surface of soybean (Glycine max). Antonie van Leeuwenhoek. 118(2), Article 41. https://doi.org/10.1007/s10482-024-02051-y